Antifungal Compound from the Predatory Bacterium Lysobacter enzymogenes Inhibits a Plant Pathogenic Fungus by Targeting the AAA ATPase VpVeb1
文献类型： 外文期刊
作者： Hou, Rongxian 1 ; Li, Kaihuai 2 ; Guo, Baodian 3 ; Zhao, Yangyang 3 ; Li, Chaohui 3 ; Tang, Bao 3 ; Sun, Weibo 3 ; Wang, Bo 3 ; Chen, Wenchan 3 ; Sheng, Cong 3 ; Kan, Jialiang 4 ; Zhao, Yancun 3 ; Liu, Fengquan 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Peoples R China
2.Guizhou Univ, Coll Agr, Dept Plant Pathol, Key Lab Agr Microbiol, Guiyang 550025, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Plant Protect, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base,Minist Sci & Technol, Nanjing 210014, Peoples R China
4.Jiangsu Acad Agr Sci, Inst Pomol, Nanjing 210014, Peoples R China
关键词： HSAF; Valsa pyri; VpVeb1; AAA ATPase; virulence
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）
ISSN： 0021-8561
年卷期： 2023 年 71 卷 41 期
页码：
收录情况： SCI
摘要： Heat-stable antifungal factor (HSAF) isolated from Lysobacter enzymogenes is considered a potential biocontrol agent. However, the target of HSAF in phytopathogenic fungi remains unclear. In this study, we investigated the target of HSAF in Valsa pyri that causes fatal pear Valsa canker. Thirty-one HSAF-binding proteins were captured and identified by surface plasmon resonance (SPR) and high-performance liquid chromatography-mass spectrometry (LC-MS/MS), and 11 deletion mutants were obtained. Among these mutants, only Delta VpVEB1 showed decreased sensitivity to HSAF. Additionally, Delta VpVEB1 exhibited significantly reduced virulence in V. pyri. Molecular docking and SPR results revealed that HSAF bound to threonine 569 and glycine 570 of VpVeb1, which are crucial for AAA ATPase activity. Another study showed that HSAF could decrease the ATPase activity of VpVeb1, leading to the reduced virulence of V. pyri. Taken together, this study first identified the potential target of HSAF in fungi. These findings will help us better understand the model of action of HSAF to fungi.
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