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Microsatellite DNA markers associated with resistance to WSSV in Penaeus (Fenneropenaeus) chinensis

文献类型: 外文期刊

作者: Dong, Shirui 1 ; Kong, Jie 1 ; Meng, Xianhong 1 ; Zhang, Qingwen 1 ; Zhang, Tianshi 1 ; Wang, Rucai 2 ;

作者机构: 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Utilizat Marine Fisheries Res, Qingdao 266071, Peoples R China

2.Tianjin Univ Cornmerce, Coll Biotechnol & Food Sci, Tianjin 300134, Peoples R China

关键词: microsatellite repeats;satellite DNA;genetic markers;shrimp;shrimp culture;disease resistance;white spot syndrome;quantitative trait loci

期刊名称:AQUACULTURE ( 影响因子:4.242; 五年影响因子:4.723 )

ISSN:

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收录情况: SCI

摘要: An F family of Chinese shrimp, Penaeus (Fenneropenaeus) chinensis, was used to conduct analysis of an association between microsatellite DNA markers and resistance to WSSV (white spot syndrome virus). The total of 163 individuals from the F family was challenged with WSSV by oral infection in an indoor tank. Nested-PCR was used to test for infection of WSSV. Eighteen primer pairs were selected to genotype 80 individuals in the F family. Associations of individual marker alleles with resistance to WSSV were analysed by ANOVA. The results showed that all shrimp challenged were confirmed to have been infected by WSSV. The mean number of alleles, mean expected heterozygosity, and mean polymorphic information content were 2.50, 0.536 and 0.446, respectively. An allele at microsatellite locus RS0622 was associated with resistance to WSSV. Identification of a larger number of DNA markers linked to QTL controlling the resistance to WSSV will contribute to the application of DNA marker-assisted selection in aquacultural breeding of Chinese shrimp.

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