您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Effect of humic acid on lysozyme interaction with montmorillonite and kaolinite

文献类型： 外文期刊

作者： Li, Yan 1 ; Koopal, Luuk K. 3 ; Tan, Wenfeng 4 ; Chai, Yanjun 5 ; Chen, Yi 1 ; Wu, Chunyan 1 ; Tang, Xu 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Environm Resource & Soil Fertilizer, Hangzhou 310021, Peoples R China

2.Natl Agr Expt Stn Soil Qual, Jiaxing 314000, Peoples R China

3.Wageningen Univ & Res, Phys Chem & Soft Matter, Stippeneg 4, NL-6708 WE Wageningen, Netherlands

4.Huazhong Agr Univ, Coll Resources & Environm, Wuhan 430070, Peoples R China

5.Zhejiang Univ Sci & Technol, Sch Environm & Nat Resources, Key Lab Recycling & Ecol Treatment Waste Biomass, Hangzhou 310023, Peoples R China

关键词： Humic acid (HA); Lysozyme (LSZ); Clays; pH; Mass ratio of HA/LSZ(f); LSZ adsorption and removal

期刊名称：SCIENCE OF THE TOTAL ENVIRONMENT （ 影响因子：10.753； 五年影响因子：10.237 ）

ISSN： 0048-9697

年卷期： 2022 年 834 卷

页码：

收录情况： SCI

摘要： Humic acid (HA) as a soil natural organic matter (NOM) can participate in the interaction between proteins and clay minerals, depending on clay type, HA and protein content, and solution conditions. The effect of HA on the interaction of lysozyme (LSZ) with kaolinite (Kao) and montmorillonite (Mont) was investigated at (initial) pH(i )5 and 8. In the solutions containing both HA and LSZ, HA/LSZ complexes were formed with a net charge density depending on pH and HA/LSZ mass ratio f. LSZ adsorption on clays in the presence of HA is dominated by adsorption of HA/LSZ complexes. The HA/LSZ mass ratio (f(IEP,pHi)) at the isoelectric point (IEP) is pH dependent. At f < f(IEP,pHi) the HA/LSZ complexes are positively charged and adsorb well to the negatively charged Mont and Kao surface fractions. The adsorption levels on Mont are considerably larger than on Kao, which is mainly due to the much larger area fraction of modestly hydrophobic basal plates of Mont. The presence of HA increased the plateau adsorption of LSZ on Kao and Mont for both pHi values, and the LSZ adsorption increased with increasing HA content and pHi values due to a decreasing mutual repulsion of the bound HA/LSZ complexes. At pH(i) 8 complications arose for low initial LSZ concentrations, for f < f(IEP,pHi) the HA/LSZ complexes were only weakly positive and formed dispersed aggregates and for f > f(IEP,pHi )the HA/LSZ complexes were negative, both conditions caused relatively high equilibrium concentrations of LSZ in solution that decreased with increasing initial LSZ concentration. The present results enhance our insight in protein soil interactions for the case that clay particles are brought in contact with aqueous solutions that contain modest amounts of both NOM and protein and stress the importance of the NOM/protein mass ratio and solution pH.

  • 相关文献

[1]Effect of humic acid on lysozyme interaction with montmorillonite and kaolinite. Li, Yan,Chen, Yi,Wu, Chunyan,Tang, Xu,Li, Yan,Chen, Yi,Wu, Chunyan,Tang, Xu,Koopal, Luuk K.,Tan, Wenfeng,Chai, Yanjun. 2022

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台