Research on inner membrane complex protein 1: a novel nanovaccines against Toxoplasma gondii
文献类型： 外文期刊
作者： Fang, YunNan 1 ; Zhou, Pan 1 ; Qi, WeiYu 1 ; Yu, YanLi 3 ; Wang, XiaoJuan 1 ; Jiang, YuChen 1 ; Zhang, Li 1 ; Yu, YouLi 4 ; Wang, JianDong 4 ; Yu, ZhengQing 1 ; Liu, TingLi 2 ;
作者机构： 1.Ningxia Univ, Sch Anim Sci & Technol, Yinchuan, Peoples R China
2.Shanxi Med Univ, Dept Med Lab Sci, Fenyang Coll, Fenyang, Peoples R China
3.Ningxia Univ, Sch Econ & Management, Yinchuan, Ningxia, Peoples R China
4.Ningxia Acad Agr & Forestry Sci, Inst Anim Sci, Yinchuan, Peoples R China
关键词：
期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.6； 五年影响因子：2.7 ）
ISSN：
年卷期： 2025 年 21 卷 1 期
页码：
收录情况： SCI
摘要： Toxoplasma gondii (T. gondii) is a globally prevalent zoonotic parasite causing severe health and economic impacts. Despite decades of research, no commercial vaccine provides comprehensive protection against both acute and chronic toxoplasmosis. DNA vaccines represent a promising strategy, but their application is hindered by low delivery efficiency and limited immunogenicity. Here, we developed and evaluated pVAX1-TgIMC1-loaded PLGA and chitosan (CS) nanospheres as potential vaccine candidates. Immunization studies in mice showed that pVAX1-TgIMC1/PLGA and pVAX1-TgIMC1/CS nanospheres induced robust humoral and cellular immune responses, significantly enhancing specific IgG levels and cytokine production IFN-gamma and IL-17 compared to the naked DNA vaccine. Both nanospheres also promoted dendritic cell maturation and T-cell activation, resulting in reduced parasite burdens in cardiac tissues post-challenge. Notably, the PLGA nanospheres exhibited superior protection against acute toxoplasmosis, while CS nanospheres provided additional advantages in antigen stability and delivery. The nanospheres were non-toxic, as confirmed by biochemical markers and histopathological analysis. These findings highlight pVAX1-TgIMC1/PLGA and pVAX1-TgIMC1/CS nanospheres as promising candidates for T. gondii vaccine development, warranting further optimization and validation in broader animal models.
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