Haplotype-resolved genome assembly and genome-wide association study identifies the candidate gene closely related to sugar content and tuber yield in Solanum tuberosum
文献类型： 外文期刊
作者： Gong, Lei 1 ; Zhang, Li 2 ; Zhang, Haiwen 3 ; Nie, Fengjie 4 ; Liu, Zhenning 5 ; Liu, Xuan 4 ; Fang, Miaoquan 6 ; Yang, Wenjing 4 ; Zhang, Yu 4 ; Zhang, Guohui 1 ; Guo, Zhiqian 1 ; Zhang, Hongxia 5 ;
作者机构： 1.Acad Ningxia, Guyuan Branch, Acad Agr & Forestry Sci, 200 Yiwu Rd, Guyuan 756000, Ningxia, Peoples R China
2.Ningxia Acad Agr & Forestry Sci, 590 Huanghe East Rd, Yinchuan 750002, Ningxia, Peoples R China
3.Peking Univ, Inst Adv Agr Sci, 699 Binhu Rd, Weifang 261325, Shandong, Peoples R China
4.Ningxia Acad Agr & Forestry Sci, Res Ctr Agr Biotechnol, Ningxia Key Lab Agrobiotechnol, 590 Huanghe East Rd, Yinchuan 750002, Ningxia, Peoples R China
5.Linyi Univ, Coll Agr & Forestry Sci, Middle Sect, Shuangling Rd, Linyi 276000, Shandong, Peoples R China
6.Huazhi Biotechnol Co Ltd, 618 Heping Rd, Changsha 410016, Hunan, Peoples R China
期刊名称：HORTICULTURE RESEARCH （ 影响因子：8.5； 五年影响因子：9.1 ）
ISSN： 2662-6810
年卷期： 2025 年 12 卷 6 期
页码：
收录情况： SCI
摘要： As an important noncereal food crop grown worldwide, the genetic improvement of potato in tuber yield and quality is largely constrained due to the lacking of a high-quality reference genome and understanding of the regulatory mechanism underlying the formation of superior alleles. Here, a chromosome-scale haplotype-resolved genome assembled from an anther-cultured progeny of 'Ningshu 15', a tetraploid variety featured by its high starch content and drought resistance was presented. The assembled genome size was 1.653 Gb, with a contig N50 of approximately 1.4 Mb and a scaffold N50 of 61 Mb. The long terminal repeat assembly index score of the two identified haplotypes of 'Ningshu 15' was 11.62 and 11.94, respectively. Comparative genomic analysis revealed that positive selection occurred in gene families related to starch, sucrose, fructose and mannose metabolism, and carotenoid biosynthesis. Further genome-wide association study in 141 accessions identified a total number of 53 quantitative trait loci related to fructose, glucose, and sucrose content. Among them, a tonoplast sugar transporter encoding gene, StTST2, closely associated with glucose content was identified. Constitutive expression of StTST2 in potato and Arabidopsis increased the photosynthetic rate, chlorophyll and sugar content, biomass tuber and seed production in transgenic plants. In addition, co-immunoprecipitation assays demonstrated that StTST2 directly interacted with SUT2. Our study provides a high-quality genome assembly and new genetic locus of potato for molecular breeding.
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