TLR1 in Nile tilapia: The conserved receptor cannot interact with MyD88 and TIRAP but can activate NF-kappa B in vitro
文献类型： 外文期刊
作者： Gao, Feng-Ying 1 ; Zhou, Xin 1 ; Lu, Mai-Xin 1 ; Wang, Miao 1 ; Liu, Zhi-Gang 1 ; Cao, Jian-Meng 1 ; Ke, Xiao-Li 1 ; Yi, Meng-Meng 1 ; Qiu, Deng-Gao 2 ;
作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat, Minist Agr, Guangzhou 510380, Peoples R China
2.Fisheries Res Inst Fujian, Key Lab Cultivat & High Value Utilizat Marine Org, Xiamen 361013, Fujian, Peoples R China
3.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangdong Prov Key Lab Aquat Anim Immune Technol, Guangzhou 510380, Peoples R China
4.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
关键词： TLR1; Nile tilapia (Oreochromis niloticus); Expression pattern; Signalling pathway; MyD88; TIRAP
期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 2021影响因子：3.605； 五年影响因子：3.833 ）
ISSN： 0145-305X
年卷期： 2022 年 127 卷
收录情况： SCI
摘要： Toll-like receptors (TLRs) play a critical role in the innate immune response of fish. In this study, we isolated the cDNA sequence of Nile tilapia TLR1 (OnTLR1). The deduced OnTLR1 protein contains a signal peptide, 7 leucine-rich repeats (LRRs), a C-terminal LRR (LRR-CT), a transmembrane region and a highly conserved TIR domain. In healthy Nile tilapia, the OnTLR1 transcript was broadly expressed in all examined tissues, with the highest expression levels in the spleen. After infection with Streptococcus agalactiae, the OnTLR1 transcripts were upre-gulated in the gill and kidney. After stimulation with polyinosinic-polycytidylic acid (poly(I:C)), the expression levels of OnTLR1 were significantly downregulated in the intestine, whereas OnTLR1 transcripts were significantly upregulated in the kidney. After challenge with lipopolysaccharide (LPS), the expression levels of OnTLR1 were significantly upregulated in the spleen and kidney. The subcellular localization showed that OnTLR1 was expressed in the cytoplasm. TLR1 significantly increased MyD88-dependent NF-kappa B activity. However, the results of a pull-down assay showed that OnTLR1 did not interact with MyD88 or TIRAP. Binding assays revealed the specificity of OnTLR1 for pathogen-associated molecular patterns (PAMPs) and bacteria that included S. agalactiae, Aeromonas hydrophila and poly(I:C) and LPS. Taken together, these findings suggest that OnTLR1, as a pattern recognition receptor (PRR), might play an important role in the immune response to pathogen invasion.
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