Purified Astragalus Polysaccharide Combined with Inactivated Vaccine Markedly Prevents Infectious Haematopoietic Necrosis Virus Infection in Rainbow Trout (Oncorhynchus mykiss)
文献类型： 外文期刊
作者： Pan, Yucai 1 ; Liu, Zhe 1 ; Quan, Jinqiang 1 ; Gu, Wei 2 ; Wang, Junwei 3 ; Zhao, Guiyan 1 ; Lu, Junhao 1 ; Wang, Jianfu 1 ;
作者机构： 1.Gansu Agr Univ, Coll Anim Sci & Technol, Lanzhou 730070, Peoples R China
2.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Minist Agr & Rural Affairs, Key Lab Freshwater Aquat Biotechnol & Breeding, Harbin 150070, Peoples R China
3.Shandong Wanzefeng Ocean Dev Grp Co Ltd, Rizhao 276800, Peoples R China
关键词： adjuvant activity; infectious hematopoietic necrosisvirus; purified Astragalus polysaccharide; vaccine
期刊名称：ACS BIOMATERIALS SCIENCE & ENGINEERING （ 影响因子：5.5； 五年影响因子：5.5 ）
ISSN： 2373-9878
年卷期： 2024 年 10 卷 11 期
页码：
收录情况： SCI
摘要： Rainbow trout (Oncorhynchus mykiss) is experiencing a catastrophic pandemic. In recent years, infectious hematopoietic necrosis virus (IHNV) has spread nationwide, resulting in significant mortality. Currently, there are no available treatments or vaccines for IHNV in China. Here, the Astragalus extract was purified and characterized. Then, we developed an inactivated IHNV vaccine with purified Astragalus polysaccharide (P-APS) as an adjuvant. Safety assays showed that IHNV was successfully inactivated. After a serious IHNV challenge, the cumulative mortality rates were 76.0, 38.0, and 22.1% in control, vaccine, and P-APS + vaccine groups, respectively. P-APS + vaccine was effective at reducing head kidney damage and apoptosis after IHNV challenge by histopathological and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) analyses. The P-APS + vaccine group showed better results in enhancing specific antibodies (IgM) and immune enzyme activities (C3, LZM, GOT, and GPT). RNA-seq revealed that many immune-related pathways were significantly enriched. TLR2, TLR7, C3, IFN-gamma, IgM, MHC1, MHC2, MX1, and VIG1 were identified as core genes based on RNA-seq and PPI networks. Mechanistic investigations showed that P-APS + vaccine activates the immune pathway by upregulating the expression of these genes. P-ASP+vaccine induced effective innate and adaptive immune responses that were stronger than single vaccines after vaccination and IHNV challenged. Our findings will provide a promising vaccine candidate against IHNV.
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