C4-HSL drives rapid biofilm formation in low-temperature aquaculture effluent: strengthening structural stability of biofilm and improving nitrogen removal efficiency
文献类型： 外文期刊
作者： Li, Junlin 1 ; Ma, Jiahao 1 ; Li, Zhifei 1 ; Xie, Jun 1 ; Zhang, Yuting 1 ; Yu, Mengyuan 1 ; Xia, Yun 1 ; Gong, Wangbao 1 ; Zhang, Kai 1 ; Wang, Guangjun 1 ; Zhong, Quanfa 1 ; Tian, Jingjing 1 ; Li, Hongyan 1 ; Xie, Wenping 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat, Guangzhou 510380, Peoples R China
2.South China Univ Technol, Sch Environm & Energy, Guangzhou 510006, Guangdong, Peoples R China
3.Guangdong Ecol Remediat Aquaculture Pollut Res Ctr, Guangzhou 510380, Peoples R China
关键词： Quorum sensing; Biofilm; Aquaculture; Nitrogen metabolism
期刊名称：ENVIRONMENTAL RESEARCH （ 影响因子：7.7； 五年影响因子：7.7 ）
ISSN： 0013-9351
年卷期： 2025 年 285 卷
页码：
收录情况： SCI
摘要： During the centralized discharge of winter aquaculture wastewater, low temperature (<= 15 degrees C) and low C/N ratio (<= 5) result in delayed initiation of the biofilm process and decreased nitrogen removal efficiency.The quorum sensing (QS) communication system serves as the core mechanism regulating biofilm formation. This study examined how adding N-butyyl-L-homo-serine lactone (C4-HSL) affects the start-up and nitrogen removal in sequential batch biofilm reactors (SBBR) operated at 14 degrees C with a C/N <= 5. Biofilm adhesion tests showed that C4-HSL levels between 400 and 1200 ng/L notably boosted initial adhesion, peaking at 700 ng/L with a 57.5 % increase over the control. The SBBR results indicated that a high concentration of C4-HSL (700-1000 ng/L) effectively removed TN and COD, achieving a TN removal rate of 91.62 %, which was three times that of the control. C4-HSL enhances low-temperature biofilm nitrogen removal by remodeling the structure of functional microbial communities and enriching bacteria along with functional genes involved in biofilm formation and nitrification-denitrification. Additionally, C4-HSL specifically upregulated the abundance of flagella assembly and biofilm matrix synthesis genes (such as virD4, yegE) and quorum sensing-related genes (such as rpfB, bapA), promoting the secretion of extracellular polymeric substances. This drove the transition of biofilm structure from a loose state to a highly organized one, accelerating the maturation of biofilm adhesion and prolonging the stabilization period of the biofilm by 15-20 days. This study revealed the influence of C4-HSL on the initiation mechanism of low-temperature biofilm process from the perspective of quorum sensing regulation, providing a theoretical basis and technical pathway for developing aquaculture effluent treatment technologies based on quorum sensing regulation.
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