您好，欢迎访问贵州省农业科学院 机构知识库！
贵州省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Cloning, characterisation and salt tolerance analysis of EuHPPa1 gene, encoding inorganic pyrophosphatase in Eucommia ulmoides Oliv

文献类型： 外文期刊

作者： Gao, Linxin 1 ; Chen, Xi 1 ; Zhao, Degang 1 ;

作者机构： 1.Guizhou Univ, Inst Agrobioengn, Coll Life Sci, Coll Tea Sci,Key Lab Plant Resources Conservat & G, Guiyang 550025, Peoples R China

2.Guizhou Acad Agr Sci, Plant Conservat Technol Ctr, Guizhou Key Lab Agr Biotechnol, Guiyang 550006, Peoples R China

关键词： E. ulmoides; Inorganic pyrophosphatase; Cloning; Expression; Salt stress

期刊名称：PLANT GROWTH REGULATION （ 影响因子：3.9； 五年影响因子：3.8 ）

ISSN： 0167-6903

年卷期： 2025 年 105 卷 4 期

页码：

收录情况： SCI

摘要： Eucommia ulmoides Oliver is dioecious plant that is prized for its Eucommia gum-rich pericarp, leaves, and barks, which makes it a valuable source of collagen with adaptability across various regions. It not only has significant medicinal value but also has advantageous traits such as drought and salt tolerance. Membrane-bound pyrophosphatase functions as an H+ proton pump, and its related genes have been successfully cloned in various plant species. The study focused on investigating the functional role of EuHPPa1 in E. ulmoides using gene cloning, bioinformatics analysis, RT-PCR, subcellular localization, transformation methods, and other approaches. The EuHPPa1 gene with 2298 bp was successfully cloned, showcasing significant advancements in understanding plant stress resistance, growth, and development. The protein encoded by the gene is present in both the cell membrane and the nuclear membrane. Analysis of the spatio-temporal expression pattern of the EuHPPa1 gene indicated a degree of tissue-specificity, with predominant expression in the roots. The promoter of the EuHPPa1 gene was cloned to predict its response elements including GA(3), ABA, MeJA, and light. Through tobacco transformation, the highest expression was observed in the root, indicating regulation by the promoter. Wild-type tobacco and transgenic tobacco were both exposed to salt stress treatment, with subsequent analysis of protective enzyme activities and stress-induced gene expression levels. Consequently, this study aimed to explore the response of EuHPPa1 to salt stress in E. ulmoides by evaluating alterations in growth and physiological indicators. Furthermore, we confirmed the gene's function and showed how the EuHPPa1 gene affects the plant's response to salt stress, which may improve the plant's ability to withstand salt stress.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：贵州省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台