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Dual-mode sensor based on the synergy of magnetic separation and functionalized probes for the ultrasensitive detection of Clostridium perfringens

文献类型： 外文期刊

作者： Wang, Wenzhuo 1 ; Yuan, Wei 2 ; Wang, Debao 5 ; Mai, Xutao 2 ; Wang, Daoying 2 ; Zhu, Yongzhi 2 ; Liu, Fang 2 ; Sun, Zhilan 2 ;

作者机构： 1.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China

2.Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety State Key Lab, Nanjing 210014, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Agr Prod Proc, Nanjing 210014, Peoples R China

4.Minist Agr & Rural Affairs, Key Lab Cold Chain Logist Technol Agra Prod, Beijing, Peoples R China

5.Inner Mongolia Acad Agr & Anim Husb Sci, Inst Agr & Livestock Prod Proc, Hohhot 010031, Peoples R China

期刊名称：RSC ADVANCES （ 影响因子：4.036； 五年影响因子：3.748 ）

ISSN：

年卷期： 2022 年 12 卷 39 期

页码：

收录情况： SCI

摘要： Clostridium perfringens is an important foodborne pathogen, which has caused serious public health problems worldwide. So, there is an urgent need for rapid and ultrasensitive detection of C. perfringens. In this paper, a dual-mode sensing platform using the synergy between fluorescent and electrochemical signals for Clostridium perfringens detection was proposed. An electrochemical aptasensor was constructed by a dual-amplification technology based on a DNA walker and hybridization chain reaction (HCR). When the C. perfringens genomic DNA was present, it specifically bonded with FAM-labeled aptamer which triggered the DNA walker on hairpin DNA (hDNA) tracks to start the synthesis of double-stranded DNA. HCR occurred subsequently and produced long-chain DNA to absorb more methylene blue (MB). In this cycle, the fluorescent signals of released FAM-labeled aptamer could also be detected. The synergistic effects of MB and FAM significantly improved the sensitivity and accuracy of the dual-mode sensor. As a result, the biosensor displayed an excellent analytical performance for C. perfringens at a concentration of 1 to 10(8) CFU g(-1). A minimum concentration of 1 CFU g(-1) and good accuracy were detected in real samples. The proposed ultrasensitive detection method for detecting C. perfringens in food showed great potential in controlling foodborne diseases.

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