Molecular characterization and antibacterial immunity functional analysis of the antimicrobial peptide hepcidin from Coregonus ussuriensis berg
文献类型： 外文期刊
作者： Liu, Enhui 1 ; Huang, Tianqing 1 ; Gu, Wei 1 ; Wang, Gaochao 1 ; Dong, Fulin 1 ; Ma, Haibing 1 ; Zhang, Lanlan 2 ; He, Xianchen 3 ; Yao, Zuochun 1 ; Jiao, Wenlong 4 ; Li, Chunyu 5 ; Wang, Bingqian 1 ; Xu, Gefeng 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Minist Agr & Rural Affairs, Key Lab Freshwater Aquat Biotechnol & Breeding, Harbin, Peoples R China
2.Heilongjiang Fisheries Technol Extens Ctr, Harbin, Peoples R China
3.Heilongjiang Aquat Anim Resource Conservat Ctr, Harbin, Peoples R China
4.Gansu Fisheries Res Inst, Lanzhou, Peoples R China
5.Xinjiang Tianyun Organ Agr Ltd Liabil Co, Yili, Peoples R China
关键词： Coregonus ussuriensis Berg; Hepcidin; Antimicrobial peptides; Functional characterization
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.622； 五年影响因子：4.799 ）
ISSN： 1050-4648
年卷期： 2022 年 122 卷
页码：
收录情况： SCI
摘要： Antimicrobial peptides are immune system molecules existing in different organisms including mollusks, crustaceans and vertebrates. Hepcidins are a group of cysteine rich antimicrobial peptides, which plays an important role in fish response to a variety of pathogens. In this study, we cloned and identified Hepcidin from the Coregonus ussuriensis Berg, and its functions in vivo and in vitro was investigated. Our results showed that, CuHepc contains a 267 bp coding sequence (CDS) region that encodes 88 putative amino acids with a molecular weight of 9.77 kD. Hepcidin transcripts were most abundant in the liver of healthy C. ussuriensis Berg. The synthesized Hepcidin peptide exhibited a wide range of antibacterial activity against Gram-positive and Gram-negative bacteria in vitro, and the results of in vivo bacterial attack assays showed that the CuHepc gene was differentially up regulated in the six tissues investigated after infection with Aeromonas hydrophila. To analyze the changes in protein levels in C. ussuriensis, we generated Hepc polyclonal antibodies in rabbits and verified that the protein expression was increased after bacterial infection with Western blot assay. MIC assay results showed a geometric mean value of 5.513 mu M for CuHepc peptide. In the in vivo experiment, immune-related genes IL-10, NF -KB, TLR3 were up-regulated post-infection CuHepc peptide in liver and intestine. Finally, CuHepc peptide reduced the tissues microbial load compared to infection with Aeromonas hydrophila. The above results indicate that Hepc plays a role in the immune response of C. ussuriensis to exogenous disturbances, indicate that CuHepc might act a candidate for modulation of the innate immune system in C. ussuriensis.
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