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Construction and efficacy of a new live chimeric C-strain vaccine with DIVA characteristics against classical swine fever

文献类型： 外文期刊

作者： Yi, Weicheng 1 ; Wang, Hao 1 ; Qin, Huan 1 ; Wang, Qin 2 ; Guo, Rui 3 ; Wen, Guoyuan 3 ; Pan, Zishu 1 ;

作者机构： 1.Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Peoples R China

2.China Inst Vet Drug Control, World Org Anim Hlth Reference Lab Class Swine Feve, Beijing 100081, Peoples R China

3.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Key Lab Prevent & Control Agents Anim Bacteriosis, Hubei Prov Key Lab Anim Pathogen Microbiol,Minist, Wuhan 430064, Peoples R China

4.Huazhong Univ Sci & Technol, Wuhan Childrens Hosp, Tongji Med Coll, Lab, Wuhan 430015, Peoples R China

关键词： Classical swine fever virus; Chimeric virus; Marker vaccine; E2; UTR; DIVA

期刊名称：VACCINE （ 影响因子：5.5； 五年影响因子：4.3 ）

ISSN： 0264-410X

年卷期： 2023 年 41 卷 12 期

页码：

收录情况： SCI

摘要： To develop the new classical swine fever (CSF) vaccine candidate with differentiating infected vaccinated animals (DIVA) characteristics, a chimeric CSF virus (CSFV) was constructed based on an infectious cDNA clone of the CSF vaccine C-strain. The 5'-and 3'-untranslated regions (UTRs) and partial E2 region (resi-dues 690-860) of the C-strain were substituted with the corresponding regions of bovine viral diarrhoea virus (BVDV) to construct the chimeric cDNA clone pC/bUTRs-tE2. The chimeric virus rC/bUTRs-tE2 was generated by several passages of pC/bUTRs-tE2-transfected PK15 cells. Stable growth and genetic prop-erties of rC/bUTRs-tE2 were obtained after 30 serial passages. Compared to parental rC/bUTRs-tE2 (1st passage), two residue mutations (M834K and M979K) located in E2 in rC/bUTRs-tE2 P30 were observed. Compared to the C-strain, rC/bUTRs-tE2 exhibited unchanged cell tropism and decreased plaque-forming ability. Substituting the C-strain UTRs with the BVDV UTRs resulted in significantly increased viral repli-cation in PK15 cells. Compared to CSFV Erns-positive and BVDV tE2-negative antibody responses induced by the CSF vaccine C-strain, immunization of rabbits and piglets with rC/bUTRs-tE2 resulted in serological profiles of CSFV Erns- and BVDV tE2-positive antibodies, which are used to serologically discriminate pigs that are clinically infected and vaccinated. Vaccination of piglets with rC/bUTRs-tE2 conferred complete protection against lethal CSFV challenge. Our results suggest that rC/bUTRs-tE2 is a promising new CSF marker vaccine candidate. (c) 2023 Elsevier Ltd. All rights reserved.

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