Construction and bacteriostatic effect analyses of a recombinant thermostable Newcastle disease virus expressing cecropin AD
文献类型： 外文期刊
作者： Yao, Lun 1 ; Ren, Xiangfei 1 ; Zhou, Ping 4 ; Yang, Junjie 1 ; Zeng, Chi 5 ; Zeng, Zhe 1 ; Shang, Yu 1 ; Feng, Helong 1 ; Jin, Mengyun 1 ; Xiao, Qianni 1 ; Shao, Huabin 1 ; Luo, Qingping 1 ; Hu, Sishun 2 ; Wen, Guoyuan 1 ;
作者机构： 1.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Hubei Prov Key Lab Anim Pathogen Microbiol, Wuhan 430064, Peoples R China
2.Huazhong Agr Univ, Coll Vet Med, Wuhan 430064, Peoples R China
3.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Key Lab Prevent & Control Agents Anim Bacteriosis, Minist Agr & Rural Affairs, Wuhan 430064, Peoples R China
4.Hubei Inst Vet Durg Control, Wuhan 430064, Peoples R China
5.Wuhan Polytech Univ, Sch Life Sci & Technol, Wuhan, Peoples R China
6.Hubei Hongshan Lab, Wuhan 430064, Peoples R China
关键词： Newcastle disease virus; Cecropin AD; Staphylococcus aureus; Wound healing
期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：2.7； 五年影响因子：2.9 ）
ISSN： 0378-1135
年卷期： 2025 年 302 卷
页码：
收录情况： SCI
摘要： Cecropin AD (CAD), a hybrid antimicrobial peptide composed of the first 11 residues of cecropin A and last 26 residues of cecropin D, is a promising antibiotic candidate. Therefore, an efficient and convenient method for producing CAD is necessary for commercial applications. The Newcastle disease virus (NDV) has been widely used as a platform for gene delivery and exogenous protein expression. In this study, we constructed a recombinant NDV that expresses CAD. To obtain high expression of the CAD peptide, tandem repeats of the cad gene were inserted into the genomes of the thermostable NDV strain TS09-C using reverse genetic technology. The thermostable recombinant NDV, namely rTS-CAD3, showed thermostability and growth kinetics similar to those of their parental strain. A bacteriostatic test showed that rTS-CAD3 inhibited Staphylococcus aureus (gram-positive bacteria) and Escherichia coli (gram-negative bacteria) in vitro. We further determined the bacteriostatic effects of rTS-CAD3 expressed CAD against S. aureus in skin wound infections. The results showed that rTS-CAD3 subcutaneously injection improved wound healing and reduced S. aureus decolonization. In summary, our results indicate that the rTS-CAD3 expressing CAD peptide is a potent antimicrobial agent against S. aureus and E. coli and may be applied to accelerate wound healing in farm animals.
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