您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Protein metabolism and immune response levels in cuttlefish (Sepia pharaonis) and the effects of inking stress on development

文献类型： 外文期刊

作者： Jiang, Maowang 1 ; Ye, Jintao 1 ; Xiao, Wencheng 1 ; Peng, Ruibing 1 ; Han, Qingxi 1 ; Chen, Siqing 2 ; Jiang, Xiamin 1 ;

作者机构： 1.Ningbo Univ, Sch Marine Sci, Key Lab Appl Marine Biotechnol, Ningbo 315832, Zhejiang Provi, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Utilizat Marine Fisheries Reso, Qingdao 266071, Peoples R China

3.Ningbo Univ, Sch Marine Sci, 818 Fenghua Rd, Ningbo 315832, Peoples R China

关键词： Physiological stress; Ink release; Nutritional composition; Protein turnover; HSP70

期刊名称：AQUACULTURE （ 影响因子：5.135； 五年影响因子：5.125 ）

ISSN： 0044-8486

年卷期： 2022 年 558 卷

页码：

收录情况： SCI

摘要： Inking is a defensive behavior that protects cephalopods from predator attack, a process that is accomplished by generating jet propulsion from powerful contractions of the mantle muscle. However, most studies have focused on the "defensive effects " of the ink, with the result that there is limited information concerning the effects of inking on the animals themselves. On the basis of our previous research, we integrated information from different tissues after continuous ink release to develop a better understanding of the physiological stress resulting from inking in the cuttlefish (Sepia pharaonis). The results showed that throughout the recovery period (60 days) of the inking treatment, the observed declines in growth performance due to decreased protein synthesis and increased energy metabolism levels were supported by a decrease in mantle muscle glycogen concentration (P < 0.01), increased hexokinase (HK) and pyruvate kinase (PK) specifical activities (P < 0.001), up-regulated expression of arginine kinase (AK) and octopine dehydrogenase (ODH) (P < 0.001), and up-regulated immune superoxide dismutase (SOD) and heat shock protein 70 (HSP70) in the digestive gland (P < 0.001). Muscle principal component analysis (PCA) revealed that amino acids (lysine, tyrosinase, arginine, leucine, isoleucine, and aspartate) were involved in protein turnover after the ink release, and essential fatty acids such as oleic acid (18:1n-9), linoleic acid (18:2n-6), arachidonic acid (ARA, 20:4n-6), and docosahexaenoic acid (DHA, 22:6n-3) were disturbed by inking stress. Moreover, the inducible HSP70 in the digestive gland was sensitively upregulated even after the 60-day recovery, and thus the transcription level of induced HSP70 in cuttlefish may be used as an indicator of physiological stress. In conclusion, a high growth rate requires a high level of protein synthesis, and it appears that considerable ink release has a significant impact on cuttlefish (S. pharaonis) physiological stress. The transcription level of HSP70 may be used as a stress indicator

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台