Effects of ultra-high pressure treatment on the protein denaturation and water properties of red swamp crayfish (Procambarus clarkia)
文献类型： 外文期刊
作者： Shi, Liu 1 ; Xiong, Guangquan 1 ; Yin, Tao 2 ; Ding, Anzi 1 ; Li, Xin 1 ; Wu, Wenjin 1 ; Qiao, Yu 1 ; Liao, Li 1 ; Jiao, Chu 1 ;
作者机构： 1.Hubei Acad Agr Sci, Inst Agr Prod Proc & Nucl Agr Technol, Hubei Innovat Ctr Agr Sci & Technol, Farm Prod Proc Res Subctr, Wuhan 430064, Peoples R China
2.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China
关键词： Red swamp crayfish; Ultra-high pressure; Myofibrillar protein; Protein denaturation; Water properties
期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：4.952； 五年影响因子：5.383 ）
ISSN： 0023-6438
年卷期： 2020 年 133 卷
页码：
收录情况： SCI
摘要： This study aimed at understanding the effects of ultra-high pressure (UHP) treatment on the quality, protein structure and water distribution of red swamp crayfish. After pressurization (100-500 MPa, 5 min), shear force, sulfhydryl (total and reactive) and carbonyl content of myofibrillar protein extracted from crayfish muscle were significantly increased (P < 0.05) with the elevated pressure, while salt soluble protein content and Ca2+-ATPase activity decreased (P < 0.05), and the secondary structure was not affected by the pressure level. After pressurization at 300 MPa, the Ca2+-ATPase activity decreased by 0.65 fold comparing with the control (P < 0.05), while the shear force, drip loss, total and reactive sulfhydryl and carbonyl content increased by 0.34, 0.32, 0.36, 0.36 and 1.56 fold, respectively (P < 0.05). Besides, SDS-PAGE of percolate displayed the decrease of actin band (42 kDa) intensity and appearance of new bands (25-40 kDa) at 300 MPa. The increased T21 (immobilized water) and T22 (free water) of samples treated at high pressure (below 400 MPa) suggested the redistribution of water compartments, which was further confirmed with the images of proton compartments. Overall, moderate UHP treatments (less than 300 MPa) could be applied to modify the protein structure and water distribution of crayfish muscle.
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