Characterization of a broad substrates specificity acyl-CoA: diacylglycerol acyltransferase 1 from the green tide alga Ulva prolifera
文献类型： 外文期刊
作者： Zhang, Xiaowen 1 ; Chi, Xiaoyuan 3 ; Wang, Yitao 1 ; Zhang, Jian 4 ; Zhang, Yan 1 ; Xu, Dong 1 ; Fan, Xiao 1 ; Liang, Che 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China
3.Shandong Peanut Res Inst, Qingdao 266100, Peoples R China
4.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Qingdao 266042, Peoples R China
关键词： Ulva prolifera; diacylglycerol acyltransferase; triacylglycerol; stress
期刊名称：ACTA OCEANOLOGICA SINICA （ 影响因子：1.431； 五年影响因子：1.445 ）
ISSN： 0253-505X
年卷期： 2020 年 39 卷 10 期
页码：
收录情况： SCI
摘要： Triacylglycerols (triglycerides, TAGs) are the major carbon and energy storage forms in various organisms, and important components of cellular membranes and signaling molecules; they have essential functions in multiple physiological processes and stress regulation. Acyl-CoA: diacylglycerol acyltransferase (DGAT) catalyzes the final and only committed acylation step in the synthesis of TAGs in eukaryotes. The present work identified and isolated a novel gene, UpDGAT1, from the green tide alga Ulva prolifera. The activity of UpDGAT1 was confirmed by heterologous expression in a Saccharomyces cerevisiae TAG-deficient quadruple mutant. Results of thin-layer chromatography and BODIPY staining indicated that UpDGAT1 was able to restore TAG synthesis and lipid body formation in the yeast. Lipid analysis of yeast cells revealed that UpDGAT1 showed broad substrate specificity, accepting saturated as well as mono- and polyunsaturated acyl-CoAs as substrates. High salinity and high temperature stresses increased UpDGAT1 expression and TAG accumulation in U. prolifera. The present study provides clues to the functions of UpDGAT1 in TAG accumulation in, and stress adaptation of, U. prolifera.
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