Applying Both Chemical Liquefaction and Enzymatic Catalysis Can Increase Production of Agaro-Oligosaccharides from Agarose
文献类型： 外文期刊
作者： Jiang, Chengcheng 1 ; Liu, Zhen 1 ; Liu, Jie 1 ; Sun, Jianan 1 ; Xu, Jiachao 1 ; Li, Laihao 3 ; Mao, Xiangzhao 1 ;
作者机构： 1.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China
3.Chinese Acad Fishery Sci, China Sea Fisheries Res Inst, Minist Agr, Key Lab Aquat Product Proc, Guangzhou 510300, Peoples R China
关键词： neoagarotetraose; agarose; agarase; marine polysaccharide; expression
期刊名称：JOURNAL OF OCEAN UNIVERSITY OF CHINA （ 影响因子：0.913； 五年影响因子：1.012 ）
ISSN： 1672-5182
年卷期： 2020 年 19 卷 6 期
页码：
收录情况： SCI
摘要： Red algae represents an important marine bioresource. One high-value utilization of red algae is the production of agaro-oligosaccharides which have many positive biological effects. However, the lack of an efficient production route seriously limits the application of agaro-oligosaccharides. In this study, we established a green route that combines chemical liquefaction and enzymatic catalysis for the efficient production of agaro-oligosaccharides, and we used the production of neoagarotetraose (NA4) as an example. Agarose (150 g L-1) liquefaction by citric acid was controlled with respect to two targets: a 100% liquefaction rate and a high average degree of polymerization (> 4) of the liquesced agaro-oligosaccharides, which were then catalyzed by beta-agarase into an oligosaccharides mixture with a high concentration of NA4 (30.8gL(-1)) in a 1-L reaction volume. After purification, we obtained 25.5g of NA4 with a purity of 92%. This work establishes an easy route for the efficient production of pure agaro-oligosaccharides from agarose.
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