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Identification and Genetic Analysis of a Novel Allelic Variation of Brittle-1 with Endosperm Mutant in Maize

文献类型： 外文期刊

作者： Wang, Sen 1 ; Zheng, Fei 1 ; Zhang, Meijing 1 ; Tu, Jun 2 ; Chen, Yanping 1 ; Yuan, Jianhua 1 ; Meng, Qingchang 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Food Crops, Prov Key Lab Agrobiol, Nanjing 210014, Peoples R China

2.Jiangsu Zhongjiang Seed Co Ltd, Nanjing 211500, Peoples R China

3.Jiangsu Collaborat Innovat Ctr Modern Crop Prod, Nanjing 210095, Peoples R China

关键词： Maize; endosperm mutant; brittle-1; gene mapping; allelic variation

期刊名称：PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：1.039； 五年影响因子：0.77 ）

ISSN： 0031-9457

年卷期： 2020 年 89 卷 4 期

页码：

收录情况： SCI

摘要： Endosperm mutants are critical to the studies on both starch synthesis and metabolism and genetic improvement of starch quality in maize. In the present study, a novel maize endosperm mutant A0178 of natural variation was used as the experimental material and identified and then characterized. Through phenotypic identification, genetic analysis, main ingredients measurement and embryo rescue, development of genetic mapping population from A0178, the endosperm mutant gene was located. The results showed that the mutant exhibited extremely low germination ability as attributed to the inhibited embryo development, and amounts of sugars were accumulated in the mutant seeds and more sugars content was detected at 23 days after pollination (DAP) in A0178 than B73. Employing genetic linkage analysis, the mutant trait was mapped in the bin 5.04 on chromosome 5. Sequence analysis showed that two sites of base transversion and insertion presented in the protein coding region and non-coding region of the mutant brittle-1 (bt1), the adenylate translocator encoding gene involved in the starch synthesis. The single base insertion in the coding region cause frameshift mutation, early termination and lose of function of Brittle-1 (BT1). All results suggested that btl is a novel allelic gene and the causal gene of this endosperm mutant, providing insights on the mechanism of endosperm formation in maize.

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