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Lysine acetylation participates in boar spermatozoa motility and acrosome status regulation under different glucose conditions

文献类型： 外文期刊

作者： Chen, Guo 1 ; Ren, Li 1 ; Chang, Zhanglin 1 ; Zhao, Yuting 1 ; Zhang, Yanwen 1 ; Xia, Dong 2 ; Zhao, Ruqian 1 ; He, Bin 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Vet Med, Key Lab Anim Physiol & Biochem, Minist Agr & Rural Affairs, Nanjing 210095, Peoples R China

2.Shanghai Acad Agr Sci, Shanghai Engn Res Ctr Breeding Pig, Shanghai 201106, Peoples R China

3.Nanjing Agr Univ, Coll Vet Med, MOE Joint Int Res Lab Anim Hlth & Food Safety, Nanjing 210095, Peoples R China

关键词： Boar spermatozoa; Semen preservation; Lysine acetylation; Spermatozoa motility

期刊名称：THERIOGENOLOGY （ 影响因子：2.74； 五年影响因子：2.93 ）

ISSN： 0093-691X

年卷期： 2021 年 159 卷

页码：

收录情况： SCI

摘要： Efficient artificial insemination (AI) with liquid preserved boar semen is essential for the swine industry. Glucose is the most widely utilized energy source in refrigerated boar semen extenders. However, the relationship between glucose concentration and spermatozoa quality is not fully understood. In the present study, boar spermatozoa were used as a model to investigate the impact of different glucose concentrations on spermatozoa motility, mitochondrial activity, and acrosome integrity at physiological temperature (37 degrees C) and during refrigeration (17 degrees C). The proportion of progressively motile spermatozoa and mitochondrial activity in the high glucose group were significantly lower than in the low glucose group when incubated at 37 degrees C for 3 h or 17 degrees C for 3 d, but not at 17 degrees C for 7 d. Lysine acetylation is a reversible post-translational modification that plays a crucial role in spermatozoa function. Our results show that spermatozoa protein acetylation levels were higher in the high glucose group than in the low glucose group. The proportions of progressively motile and acrosome-intact spermatozoa were higher in acetyltransferase inhibitor (WM-1119)-treated spermatozoa than in the control. Spermatozoa acetyl-CoA concentration, which is directly linked to acetylation, was significantly higher in the high glucose group than in the low glucose group. Taken together, spermatozoa motility and acrosome integrity can be altered by changing the concentration of glucose in the extender. High glucose concentration-induced lysine acetylation participates in the regulation of boar spermatozoa motility and acrosome integrity during preservation. These results can provide insights into spermatozoa preservation and AI in the swine industry. (C) 2020 Elsevier Inc. All rights reserved.

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