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Investigation of Sample Size Estimation for Measuring Quantitative Characteristics in DUS Testing of Shiitake Mushrooms

文献类型： 外文期刊

作者： Deng, Shan 1 ; Zhang, Meiyan 2 ; Li, Aiai 4 ; Ren, Li 1 ; Zhang, Yiying 1 ; Zhao, Hong 1 ; Zhang, Yu 1 ; Song, Chunyan 2 ; Han, Ruixi 4 ; Tan, Qi 2 ; Chu, Yunxia 1 ; Chen, Hairong 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Agrifood Stand & Testing Technol, Shanghai 201403, Peoples R China

2.Minist Agr & Rural Affairs, Shanghai Sub Ctr New Plant Variety Tests, Shanghai 201415, Peoples R China

3.Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai 201403, Peoples R China

4.Minist Agr & Rural Affairs, Dev Ctr Sci & Technol, Beijing 100176, Peoples R China

关键词： sample size; quantitative characteristics; DUS

期刊名称：AGRONOMY-BASEL （ 影响因子：3.3； 五年影响因子：3.7 ）

ISSN：

年卷期： 2024 年 14 卷 6 期

页码：

收录情况： SCI

摘要： The sampling technique is commonly used in research investigations to more accurately estimate data with greater precision, at a lower cost and in less time. In plant DUS (distinctness, uniformity, and stability) testing, many quantitative characteristic data usually need to be obtained through individual measurements. However, there is currently no scientific method for determining the appropriate sampling size. The minimum number of testing samples for DUS testing was calculated based on the theory of sample size in descriptive studies and was validated through simple random sampling. The results show that the quantitative characteristics for the edible mushroom Shiitake (Lentinula edodes) in DUS testing were uniform. The calculated results show that 10 fruiting bodies for a single measurement were sufficient. Furthermore, the outcomes of the random sampling revealed that the mean of 10 samples did not significantly differ from the mean of all data. When the sample size exceeded 10, Cohen's kappa statistic suggested that the conclusion of distinctness was very close to the near-perfect agreement. Reducing the number of samples did not affect the uniform assessment. This study suggests that the theory of sample size in descriptive studies could be applied to calculate the minimum sample size in DUS testing, and for Shiitake DUS testing, measuring 10 fruiting bodies was sufficient.

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