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Detecting Cynoglossus semilaevis infected with Vibrio harveyi using micro RNAs from mucous exosomes

文献类型： 外文期刊

作者： Zhao, Na 1 ; Zhang, Bo 2 ; Xu, Zihui 1 ; Jia, Lei 2 ; Li, Ming 4 ; He, Xiaoxu 2 ; Bao, Baolong 1 ;

作者机构： 1.Shanghai Ocean Univ, Key Lab Explorat & Utilizat Aquat Genet Resources, Int Res Ctr Marine Biosci, Minist Educ,Minist Sci & Technol,Natl Demonstrat, Shanghai 201306, Peoples R China

2.Tianjin Fisheries Res Inst, Tianjin, Peoples R China

3.Tianjin Haolingsaiao Biotechnol Co Ltd, Tianjin, Peoples R China

4.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Beijing, Peoples R China

关键词： Micro RNAs; Mucous exosome; Cynoglossus semilaevis; Vibrio harveyi; Biomarkers

期刊名称：MOLECULAR IMMUNOLOGY （ 影响因子：4.407； 五年影响因子：4.227 ）

ISSN： 0161-5890

年卷期： 2020 年 128 卷

页码：

收录情况： SCI

摘要： Exosomes are important mediators of vesicle transportation and contain microRNAs (miRNAs) that mediate transcriptional gene knockout and silencing in biological processes. Moreover, exosomic miRNAs are promising biomarkers for disease diagnosis and physiological status indication in many species, including fish. The impact of the Vibrio harveyi pathogen on Cynoglossus semilaevis aquaculture is becoming more and more serious as the industry expands. To overcome this challenge, miRNAs in mucous exosomes were screened by small RNA sequencing and verified by quantitative real-time PCR to develop biomarkers. This is the first capture of exosomes from flatfish mucus coupled with miRNA profiling. The results revealed significant differences in expression levels of some miRNAs between infected and healthy fish. Three unique miRNAs were identified for V. harveyi infection diagnosis; expression levels of dre-miR-205-5p and dre-miR-205-5p in infected fish were significantly lower than controls, while dre-miR-100-5p expression was higher. These miRNAs in mucous exosomes could be used to differentiate diseased and healthy fish in an early screening method with practical value for breeding disease-resistant C. semilaevis.

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