Quantitative Proteomic Analysis of Global Protein Acetylation in PRRSV-Infected Pulmonary Alveolar Macrophages
文献类型： 外文期刊
作者： Fang, Jianyu 1 ; Qiao, Songlin 2 ; Wang, Keling 1 ; Li, Rui 2 ; Wang, Lei 2 ; Li, Haili 1 ; Zhang, Gaiping 2 ;
作者机构： 1.Henan Acad Agr Sci, Inst Anim Husb & Vet Sci, Zhengzhou 450002, Peoples R China
2.Henan Acad Agr Sci, Key Lab Anim Immunol, Minist Agr, Zhengzhou 450002, Peoples R China
3.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China
关键词： acetylation; liquid chromatography‐ mass spectrometry; porcine reproductive and respiratory syndrome; post‐ translational modification; tandem mass tag
期刊名称：PROTEOMICS （ 影响因子：3.984； 五年影响因子：3.831 ）
ISSN： 1615-9853
年卷期： 2021 年 21 卷 2 期
页码：
收录情况： SCI
摘要： Porcine reproductive and respiratory syndrome (PRRS), caused by PRRS virus (PRRSV), is a serious viral disease affecting global swine industry. Due to the lack of effective vaccines, new antiviral strategies to compensate for the inefficacy of available vaccines are urgently required. Lysine acetylation, as an important post-translational modification during infection, plays a key regulatory role in host antiviral responses. In this study, the global acetylome is profiled using acetylation specific antibody-based enrichment and tandem mass tag label high-affinity purification liquid chromatography-mass spectrometry in PRRSV-infected pulmonary alveolar macrophages (PAMs). As a result, 3731 lysine acetylation sites on 1421 cellular proteins are identified. Bioinformatics analysis of the different acetylated proteins revealed their involvement in various biological processes, including the host immune response and energy metabolism. These findings will contribute to the understanding of PRRSV pathogenesis and identify new cellular targets for anti-PPRSV therapeutics.
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