Full-length transcriptome sequencing and modular organization analysis of oleanolic acid- and dammarane-type saponins related gene expression patterns in Panax japonicus
文献类型： 外文期刊
作者： Xu, Ran 1 ; Zhang, Jiao 1 ; You, Jingmao 2 ; Gao, Limei 3 ; Li, Yongchang 4 ; Zhang, Shaopeng 1 ; Zhu, Wenjun 1 ; Shu, Sh 1 ;
作者机构： 1.Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China
2.Hubei Acad Agr Sci, Inst Chinese Herbal Med, Enshi 445000, Peoples R China
3.South Cent Univ Nationalities, Sch Pharmaceut Sci, Wuhan 430074, Peoples R China
4.Kansas City Univ Med & Biosci, Joplin, MO 64804 USA
5.Huazhong Agr Univ, Sch Plant Sci & Technol, Wuhan 430070, Peoples R China
关键词： Panax japonicus; Saponin distribution; Biosynthesis; Rhizoma and root tissues; Metabolome; Full-length transcriptome
期刊名称：GENOMICS （ 影响因子：5.736； 五年影响因子：4.939 ）
ISSN： 0888-7543
年卷期： 2020 年 112 卷 6 期
页码：
收录情况： SCI
摘要： The saponins found in Panax japonicus, a traditional medicinal herb in Asia, exhibit high degrees of structural and functional similarity. In this study, metabolite analysis revealed that oleanolic acid-type and dammaranetype saponins were distributed unevenly in three tissues (rhizome_Y, rhizome_O, and secRoot) of P. japonicus. Single-molecule real-time (SMRT) sequencing and next generation sequencing (NGS) data revealed distinct and tissue-specific transcriptomic patterns relating to the production of these two types of saponins. In the co -expression network and hierarchical clustering analyses, one 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) and two 1-deoxy-D-xylulose-5-phosphate synthase (DXS) etc. transcripts were found to be key genes associated with the biosynthesis of oleanolic acid and dammarane-type saponins in P. japonicus, respectively. In addition, cytochrome p450 (CYP) and UDP-glucuronosyltransferase (UGT) family proteins that serve as regulators of saponin biosynthesis-related genes were also found to exhibit tissue-specific expression patterns. Together these results offer a comprehensive metabolomic and transcriptomic overview of P. japonicus.
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