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Proteomic and Metabolomic Analyses Provide Insights into the Mechanism on Arginine Metabolism Regulated by tRNA Modification Enzymes GidA and MnmE of Streptococcus suis

文献类型： 外文期刊

作者： Gao, Ting 1 ; Yuan, Fangyan 1 ; Liu, Zewen 1 ; Liu, Wei 1 ; Zhou, Danna 1 ; Yang, Keli 1 ; Guo, Rui 1 ; Liang, Wan 1 ; Zou, 1 ;

作者机构： 1.Hubei Acad Agr Sci, Key Lab Prevent & Control Agents Anim Bactaiosis, Hubei Prov Key Lab Anim Pathogen Microbiol, Minist Agr & Rural Affairs,Inst Anim Husb & Vet, Wuhan, Peoples R China

2.Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan, Peoples R China

3.Cooperat Innovat Ctr Sustainable Pig Prod, Wuhan, Peoples R China

关键词： Streptococcus suis; GidA-MnmE tRNA modification pathway; TMT; arginine metabolism; ADS; growth; pathogenicity

期刊名称：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY （ 影响因子：5.293； 五年影响因子：5.882 ）

ISSN： 2235-2988

年卷期： 2020 年 10 卷

页码：

收录情况： SCI

摘要： GidA and MnmE, two important tRNA modification enzymes, are contributed to the addition of the carboxymethylaminomethyl (cmnm) group onto wobble uridine of tRNA. GidA-MnmE modification pathway is evolutionarily conserved among Bacteria and Eukarya, which is crucial in efficient and accurate protein translation. However, its function remains poorly elucidated in zoonotic Streptococcus suis (SS). Here, a gidA and mnmE double knock-out (DKO) strain was constructed to systematically decode regulatory characteristics of GidA-MnmE pathway via proteomic. TMT labelled proteomics analysis identified that many proteins associated with cell divison and growth, fatty acid biosynthesis, virulence, especially arginine deiminase system (ADS) responsible for arginine metabolism were down-regulated in DKO mutant compared with the wild-type (WT) SC19. Accordingly, phenotypic experiments showed that the DKO strain displayed decreased in arginine consumption and ammonia production, deficient growth, and attenuated pathogenicity. Moreover, targeted metabolomic analysis identified that arginine was accumulated in DKO mutant as well. Therefore, these data provide molecular mechanisms for GidA-MnmE modification pathway in regulation of arginine metabolism, cell growth and pathogenicity of SS. Through proteomic and metabolomic analysis, we have identified arginine metabolism that is the links between a framework of protein level and the metabolic level of GidA-MnmE modification pathway perturbation.

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