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Transcriptome Analysis Identified Coordinated Control of Key Pathways Regulating Cellular Physiology and Metabolism upon Aspergillus flavus Infection Resulting in Reduced Aflatoxin Production in Groundnut

文献类型： 外文期刊

作者： Soni, Pooja 1 ; Nayak, Spurthi N. 3 ; Kumar, Rakesh 4 ; Pandey, Manish K. 1 ; Singh, Namita 1 ; Sudini, Hari K. 5 ; Ba 1 ;

作者机构： 1.Int Crops Res Inst Semi Arid Trop, Ctr Excellence Genom & Syst Biol CEGSB, Hyderabad 502324, India

2.Osmania Univ, Dept Genet, Hyderabad 500007, India

3.Univ Agr Sci, Dept Biotechnol, Dharwad 580005, Karnataka, India

4.Cent Univ Karnataka, Dept Life Sci, Gulbarga 585367, India

5.Int Crops Res Inst Semi Arid Trop, Integrated Crop Improvement, Hyderabad 502324, India

6.Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Starkville, MS 39762 USA

7.Guangdong Acad Agr Sci, Guangdong Prov Key Lab Crop Genet Improvement, South China Peanut Subctr Natl Ctr Oilseed Crops, Crops Res Inst, Guangzhou 510640, Peoples R China

8.Fujian Agr & Forestry Univ FAFU, Inst Oil Crops, Fuzhou 350002, Peoples R China

9.Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan 430062, Peoples R China

10.USDA ARS, Crop Genet & Breeding Res Unit, Tifton, GA 31793 USA

关键词： Aspergillus flavus; groundnut; aflatoxin production; RNA-Seq; transcriptome analysis; groundnut improvement

期刊名称：JOURNAL OF FUNGI （ 影响因子：5.816； 五年影响因子：6.499 ）

ISSN：

年卷期： 2020 年 6 卷 4 期

页码：

收录情况： SCI

摘要： Aflatoxin-affected groundnut or peanut presents a major global health issue to both commercial and subsistence farming. Therefore, understanding the genetic and molecular mechanisms associated with resistance to aflatoxin production during host-pathogen interactions is crucial for breeding groundnut cultivars with minimal level of aflatoxin contamination. Here, we performed gene expression profiling to better understand the mechanisms involved in reduction and prevention of aflatoxin contamination resulting from Aspergillus flavus infection in groundnut seeds. RNA sequencing (RNA-Seq) of 16 samples from different time points during infection (24 h, 48 h, 72 h and the 7th day after inoculation) in U 4-7-5 (resistant) and JL 24 (susceptible) genotypes yielded 840.5 million raw reads with an average of 52.5 million reads per sample. A total of 1779 unique differentially expressed genes (DEGs) were identified. Furthermore, comprehensive analysis revealed several pathways, such as disease resistance, hormone biosynthetic signaling, flavonoid biosynthesis, reactive oxygen species (ROS) detoxifying, cell wall metabolism and catabolizing and seed germination. We also detected several highly upregulated transcription factors, such as ARF, DBB, MYB, NAC and C2H2 in the resistant genotype in comparison to the susceptible genotype after inoculation. Moreover, RNA-Seq analysis suggested the occurrence of coordinated control of key pathways controlling cellular physiology and metabolism upon A. flavus infection, resulting in reduced aflatoxin production.

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