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Analysis of Whole Transcriptome RNA-seq Data Reveals Many Alternative Splicing Events in Soybean Roots under Drought Stress Conditions

文献类型： 外文期刊

作者： Song, Li 1 ; Pan, Zhenzhi 1 ; Chen, Lin 1 ; Dai, Yi 1 ; Wan, Jinrong 2 ; Ye, Heng 2 ; Nguyen, Henry T. 2 ; Zhang, Guozhen 1 ;

作者机构： 1.Yangzhou Univ, Coinnovat Ctr Modern Prod Technol Grain Crops, Joint Int Res Lab Agr & Agri Prod Safety, Jiangsu Key Lab Crop Genom & Mol Breeding, Yangzhou 225009, Jiangsu, Peoples R China

2.Univ Missouri, Natl Ctr Soybean Biotechnol, Columbia, MO 65211 USA

3.Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA

4.Nanjing Agr Univ, Natl Ctr Soybean Improvement, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China

5.Jiangsu Acad Agr Sci, Inst Ind Crops, Nanjing 210014, Peoples R China

关键词： soybean; root; alternative splicing; drought response; transcriptome

期刊名称：GENES （ 影响因子：4.096； 五年影响因子：4.339 ）

ISSN：

年卷期： 2020 年 11 卷 12 期

页码：

收录情况： SCI

摘要： Alternative splicing (AS) is a common post-transcriptional regulatory mechanism that modulates gene expression to increase proteome diversity. Increasing evidence indicates that AS plays an important role in regulating plant stress responses. However, the mechanism by which AS coordinates with transcriptional regulation to regulate drought responses in soybean remains poorly understood. In this study, we performed a genome-wide analysis of AS events in soybean (Glycine max) roots grown under various drought conditions using the high-throughput RNA-sequencing method, identifying 385, 989, 1429, and 465 AS events that were significantly differentially spliced under very mild drought stress, mild drought stress, severe drought stress, and recovery after severe drought conditions, respectively. Among them, alternative 3 ' splice sites and skipped exons were the major types of AS. Overall, 2120 genes that experienced significant AS regulation were identified from these drought-treated root samples. Gene Ontology term analysis indicated that the AS regulation of binding activity has vital roles in the drought response of soybean root. Notably, the genes encoding splicing regulatory factors in the spliceosome pathway and mRNA surveillance pathway were enriched according to the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis. Splicing regulatory factor-related genes in soybean root also responded to drought stress and were alternatively spliced under drought conditions. Taken together, our data suggest that drought-responsive AS acts as a direct or indirect mode to regulate drought response of soybean roots. With further in-depth research of the function and mechanism of AS in the process of abiotic stress, these results will provide a new strategy for enhancing stress tolerance of plants.

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