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Maize AKIN beta gamma Proteins Interact with P8 of Rice Black Streaked Dwarf Virus and Inhibit Viral Infection

文献类型： 外文期刊

作者： Li, Mingjun 1 ; Sun, Xi 1 ; Di, Dianping 4 ; Zhang, Aihong 4 ; Qing, Ling 3 ; Zhou, Tao 1 ; Miao, Hongqin 4 ; Fan, Zaifen 1 ;

作者机构： 1.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China

2.China Agr Univ, Key Lab Pest Monitoring & Green Management MOA, Beijing 100193, Peoples R China

3.Southwest Univ, Coll Plant Protect, Chongqing Key Lab Plant Dis Biol, Chongqing 400716, Peoples R China

4.Hebei Acad Agr & Forestry Sci, Plant Protect Inst, Baoding 071000, Peoples R China

关键词： rice black streaked dwarf virus; P8 protein; ZmAKINβ γ protein interaction; viral accumulation; primary carbohydrate metabolism

期刊名称：VIRUSES-BASEL （ 影响因子：5.048； 五年影响因子：5.127 ）

ISSN：

年卷期： 2020 年 12 卷 12 期

页码：

收录情况： SCI

摘要： Rice black streaked dwarf virus (RBSDV) is an important agent causing maize rough dwarf disease, whereas the host factors responding to RBSDV infection are poorly understood. To uncover the molecular interactions between RBSDV and maize, a yeast two-hybrid screen of a maize cDNA library was carried out using the viral P8 protein as a bait. ZmAKIN beta gamma-1 and ZmAKIN beta gamma-2 (beta gamma subunit of Arabidopsis SNF1 kinase homolog in maize) possessing high sequence similarities (encoded by two gene copies) were identified as interaction partners. Their interactions with P8 were confirmed in both Nicotiana benthamiana cells and maize protoplasts by bimolecular fluorescence complementation assay. The accumulation levels of ZmAKIN beta gamma mRNAs were upregulated at the stage of the viral symptoms beginning to appear and then downregulated. ZmAKIN beta gamma s are putative regulatory subunits of the SnRK1 complex, a core regulator for energy homeostasis. Knockdown of ZmAKIN beta gamma s in maize regulated the expression levels of the genes involved in sugar synthesis or degradation, and also the contents of both glucose and sucrose. Importantly, downregulation of ZmAKIN beta gamma s expressions facilitated the accumulation of RBSDV in maize. These results implicate a role of ZmAKIN beta gamma s in the regulation of primary carbohydrate metabolism, and in the defense against RBSDV infection.

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