Characterization of a novel spexin gene (spx2) in the half-smooth tongue sole and regulation of its expression by nutritional status
文献类型： 外文期刊
作者： Wang, Bin 1 ; Cui, Aijun 1 ; Tian, Jing 4 ; Zhang, Yaxing 1 ; Jiang, Yan 1 ; Xu, Yongjiang 1 ; Liu, Xuezhou 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr & Rural Affairs, Key Lab Sustainable Dev Marine Fisheries, Qingdao 266071, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Fisheries & Food Prod Proc, Qingdao 266237, Peoples R China
3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
4.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China
5.Huazhong Agr Univ, MOE Key Lab Environm Correlat Dietol, Wuhan 430070, Peoples R China
关键词： Spexin; Starvation; Feeding control; Reproduction; Cynoglossus semilaevis
期刊名称：AQUACULTURE REPORTS （ 影响因子：3.216； 五年影响因子：3.812 ）
ISSN： 2352-5134
年卷期： 2020 年 18 卷
页码：
收录情况： SCI
摘要： Spexin (SPX) is a newly identified peptide hormone of 14 amino acids (aa) with pleiotropic functions in vertebrates, and recently a second paralog of SPX (named SPX2) has been only found in some non-mammalian species (the previous form of SPX is now designated as SPX1). Results of previous studies have revealed the existence of a functional SPX1 in the half-smooth tongue sole. To clarify the divergence of the spexin family, we further isolated the spx2 gene from the same flatfish species in the current study. The open reading frame of tongue sole spx2 was 378 nucleotides in size that encoded a 125-aa preprohormone, and the tongue sole SPX2 precursor included a 17-aa mature peptide that may be amidated at the C-terminus, rather than a tetradecapeptide reported in most other vertebrates. Tissue distribution analysis showed that spx2 transcripts were found in a wide array of tissues of both sexes with the highest expression in the ovary. In addition, the spx2 mRNA abundance was also ubiquitously detected in different brain regions. Food deprivation for 4 weeks stimulated spx2 mRNA levels at the brain-pituitary-ovary axis, and refeeding of starved fish restored the spx2 mRNA levels back to normal. Taken together, this study provides initial evidence for the existence of a novel spexin gene (spx2) in Pleuronectiformes and suggest its possible involvement in the regulation of energy balance in the female tongue sole.
- 相关文献
作者其他论文 更多>>
-
Osmoregulation and Physiological Response of Largemouth Bass (Micropterus salmoides) Juvenile to Different Salinity Stresses
作者：Liu, Yang;Tian, Jing;Song, Hongmei;Zhu, Tao;Lei, Caixia;Du, Jinxing;Li, Shengjie;Liu, Yang
关键词：largemouth bass; salinity; osmoregulation; antioxidant enzymes; tissue damage
-
In vivo effects of NPFF and NPAF peptides on gene expression in the brain-pituitary-gonadal axis of female half-smooth tongue soles
作者：Liu, Hanlin;Chen, Huapu;Liu, Hanlin;Li, Ruixue;Yu, Zhihua;Wang, Bin;Liu, Hanlin;Li, Ruixue;Yu, Zhihua;Wang, Bin;Li, Ruixue;Yu, Zhihua
关键词：Half-smooth tongue sole; NPFF; NPAF; Reproductive axis
-
Molecular cloning and expression dynamics of Atrogin-1 in muscle tissue of Russian sturgeon (Acipenser gueldenstaedtii) under high salt stress
作者：Mo, Yong-Juan;Han, Zhi-Qiang;Gao, Yi-Li;Gao, Yi-Li;Xu, Shi-Jjian;Wang, Bin;Wu, Jingping;Xu, Shi-Jjian;Wang, Bin;Wu, Jingping;Yu, Peng;Li, Ming;Kang, Xiangtao
关键词：Atrogin-1; High salt stress; Acipenser gueldenstaedtii; Selective breeding
-
Effects of leptin on fatty acid metabolism in the liver and reproductive function of tongue sole (Cynoglossus semilaevis) in vivo
作者：Cai, Xin;Li, Yuting;Meng, Zhaojun;Cui, Aijun;Jiang, Yan;Xu, Yongjiang;Meng, Zhaojun
关键词：Leptin; Tongue sole; Lipid metabolism; Regulatory mechanism; Reproductive function
-
The effects of 5-Hydroxymethylfurfural in mitigating transportation stress of large yellow croaker (Larimichthys crocea)
作者：Zheng, Jichang;Feng, Yuan;Xu, Yongjiang
关键词：Larimichthys crocea; 5-Hydroxymethylfurfural; Transportation stress; PI3K-AKT signaling pathway; Heat shock protein 90 alpha
-
Genetic diversity analysis and DNA fingerprinting of different populations of largemouth bass (Micropterus salmoides) in China with fluorescence-labeled microsatellite markers
作者：Du, Jinxing;Zhu, Tao;Tian, Taihang;Song, Hongmei;Lei, Caixia;Tian, Jing;Li, Shengjie;Han, Linqiang
关键词：Largemouth bass; Microsatellite marker; Genetic diversity; DNA fingerprinting; Identification
-
RNA-Seq Reveals Adaptation Strategy in Grass Carp (Ctenopharyngodon idella) Under Hypersaline Conditions
作者：Zhu, Tao;Song, Hongmei;Zhu, Zhu;Tian, Jing;Lei, Caixia;Du, Jinxing;Li, Shengjie
关键词：grass carp; salt tolerance; aquaculture; transcriptome
