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Comparative analysis of RNA-Seq data reveals adventitious root development is mediated by ChIFN alpha in lotus japonicus

文献类型： 外文期刊

作者： Yang, Shimei 1 ; Ma, Siyu 1 ; Qiu, Riguang 1 ; Guang, Qiao 1 ; Lv, Yun 1 ; Dong, Yaping 1 ; Wu, Jiahai 3 ; Song, Li 1 ;

作者机构： 1.Guizhou Univ, Coll Life Sci, Guizhou Key Lab Agrobioengn,Minist Educ,Inst Agro, Key Lab Plant Resource Conservat & Germplasm Inno, Guiyang 550025, Guizhou, Peoples R China

2.South China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou, Guangdong, Peoples R China

3.Guizhou Acad Agr Sci, Inst Fruit Res, Guiyang 550025, Guizhou, Peoples R China

关键词： Lotus japonicus; RNA sequencing; adventitious root; molecular network; transcription factors

期刊名称：BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT （ 影响因子：1.632； 五年影响因子：2.029 ）

ISSN： 1310-2818

年卷期： 2021 年 35 卷 1 期

页码：

收录情况： SCI

摘要： The formation of adventitious roots (ARs) plays an important role in grass growth and production. Chicken alpha interferon (ChIFN alpha) is an animal glycoprotein with broad activities of immune control, gene induction, growth and development regulation. ChIFN alpha gene was transformed to Lotus japonicus and its expression was found to promote AR development. The primordia formation occurred 3-5 days earlier, and more root hairs appeared in the transgenic plants (TP) than in the wild-type parents (WT). Data of RNA sequences from TP and WT in vitro hydroponic culture were analyzed. Overall, the intersection of the differentially upregulated genes at 0-12 days included 278 genes in TP and 244 genes in WT plants. Gene Ontology analysis indicated that the differentially expressed genes (DEGs) in TPs were enriched in response to biotic stimulus, metabolic process, sucrose transport, carbon fixation and signal transduction. Further, Kyoto Encyclopedia of Genes and Genomes enrichment analysis found carbon metabolism, carbon fixation in photosynthetic organisms and photosynthesis enriched in TPs. Co-expression analysis of the DEGs showed that 35 and 18 transcription factors (TFs) were involved in ARs formation in TP and WT plants, respectively. However, the TFs C3H and C2C2-GATA showed the opposite regulatory pattern with ChIFN alpha expression. Together, these data suggest that ChIFN alpha promotes AR development by modulating specific pathways and TFs. These results provide new insight into the mechanism by which animal interferon modulates plant growth and lays the foundation for cultivating new forage germplasm using animal cytokines.

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