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Identification of novel antioxidant peptides from snakehead (Channa argus) soup generated during gastrointestinal digestion and insights into the anti-oxidation mechanisms

文献类型： 外文期刊

作者： Zhang, Jin 1 ; Li, Mei 4 ; Zhang, Gaonan 1 ; Tian, Yu 5 ; Kong, Fanbin 3 ; Xiong, Shanbai 1 ; Zhao, Siming 1 ; Jia, Dan 7 ;

作者机构： 1.Huazhong Agr Univ, Coll Food Sci & Technol, 1 Shizishan St, Wuhan 430070, Hubei, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Food Sci, Hangzhou 310021, Zhejiang, Peoples R China

3.Univ Georgia, Dept Food Sci & Technol, Athens, GA 30602 USA

4.Chinese Acad Sci, Brain Cognit & Brain Dis Inst BCBDI, Shenzhen Inst Adv Technol, CAS Key Lab Brain Connectome & Manipulat, Shenzhen 518055, Peoples R China

5.Chinese Acad Sci, Innovat Acad Precis Measurement Sci & Technol, Wuhan Inst Phys & Math,Key Lab Magnet Resonance B, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Hubei, Peoples R China

6.Natl R&D Branch Ctr Convent Freshwater Fish Proc, Wuhan 430070, Hubei, Peoples R China

7.Yunnan Agr Univ, Coll Anim Sci & Technol, Kunming 650201, Yunnan, Peoples R China

8.Univ West London, Sch Human & Social Sci, Middlesex TW8 9GA, England

关键词： Antioxidant peptides; Snakehead soup; Gastrointestinal digestion; Molecular docking; UPLC-MS/MS; RP-HPLC; Kelch-like ECH-associated protein 1; Cytoprotective effect

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN： 0308-8146

年卷期： 2021 年 337 卷

页码：

收录情况： SCI

摘要： Antioxidant peptides obtained from snakehead (Channa argus) soup (SHS) after simulated gastrointestinal (GI) digestion were separated, identified and characterized. Results showed that the fraction with MW < 3 kDa had the highest antioxidant capacity. Four novel antioxidant peptides were identified after RP-HPLC and UPLC-MS/MS. PGMLGGSPPGLLGGSPP and SDGSNIHFPN had the highest DPPH radical scavenging activity (IC50 = 1.39 mM) and Fe2+ chelating ability (IC50 = 4.60 mM), respectively. Structures in silico for IVLPDEGK, PGMLGGSPPGLLGGSPP and SDGSNIHFPN suggest at least one beta-turn and/or alpha-helix, which are associated with antioxidant activity. Moreover, our results showed that these three peptides docked with a recombinant Kelch-like ECH-associated protein 1 (Keap1) with a binding score greater than TX6, a good ligand of Keap1. The cell viability assay also showed significant cytoprotective effects against H2O2-induced cellular oxidative damage. This information implies that antioxidant mechanisms of novel SHS peptides occurred via activation of cellular anti-oxidation Keap1-Nrf2 signaling pathway.

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