Real-time and visual detection of Cercospora canescens based on the cytb gene using loop-mediated isothermal amplification (LAMP)
文献类型： 外文期刊
作者： Liu, Zhen 1 ; Zhang, Xinchun 2 ; Chen, Di 1 ; Hou, Jumei 1 ; Liu, Tong 1 ;
作者机构： 1.Hainan Univ, Key Lab Green Prevent & Control Trop Plant Dis &, Minist Educ, Coll Plant Protect, Haikou, Hainan, Peoples R China
2.Minist Agr & Rural Affairs, Key Lab Integrated Pest Management Trop Crops, Inst Environm & Plant Protect, CATAs, Haikou, Hainan, Peoples R China
关键词： Cercospora canescens; Cytochrome b; Detection; LAMP; SYBR Green I
期刊名称：CANADIAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：2.442； 五年影响因子：1.993 ）
ISSN： 0706-0661
年卷期：
页码：
收录情况： SCI
摘要： Cercospora canescens Ellis & Martin is an important fungal pathogen that causes leaf spot and defoliation in mungbean (Vigna radiata (Linn.) Wilczek.). Molecular methods for detection of this pathogen have not yet been developed. In this study, we developed a real-time and visual loop-mediated isothermal amplification (LAMP) assay based on the cytochrome b (cytb) gene to detect C. canescens in mungbean. No cross-reactions were observed in specificity tests conducted with 10 other plant pathogenic fungal species. The minimum detectable concentration was 100 pg/mu L C. canescens genomic DNA, and the amplified product could be visualized with the addition of SYBR Green I fluorescent dye, which could make the assay more convenient for deployment in the field. The LAMP method presented provides a specific, rapid and visual diagnostic tool for the identification of C. canescens, and has the potential to be standardized as a detection method for this pathogen in the field.
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