文献类型: 外文期刊
作者: Luo, Qi 1 ; Hu, Shuzhen 1 ; Lin, Qi 1 ; Xu, Fei 2 ; Peng, Jiejun 1 ; Zheng, Hongying 1 ; Wu, Guanwei 1 ; Rao, Shaofei 1 ; Ch 1 ;
作者机构: 1.Ningbo Univ, Inst Plant Virol, State Key Lab Managing Biot & Chem Threats Qual &, Ningbo 315211, Peoples R China
2.Zhejiang Acad Agr Sci, Publ Lab, Hangzhou 310021, Peoples R China
3.Hubei Acad Agr Sci, Inst Econ Crops, Wuhan 430064, Peoples R China
期刊名称:ARCHIVES OF VIROLOGY ( 影响因子:2.574; 五年影响因子:2.466 )
ISSN: 0304-8608
年卷期: 2021 年 166 卷 3 期
页码:
收录情况: SCI
摘要: The complete genome sequence of a novel foveavirus identified in garlic (Allium sativum L.) in China was determined using RNA-seq, reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR. The entire genomic RNA (GenBank accession MT981417) is 8748 nucleotides long excluding the 3'-terminal poly(A) tail and contains five open reading frames (ORFs). These ORFs encode the viral replicase, a triple gene block, and a coat protein. The virus was tentatively named "garlic yellow stripe associated virus" (GarYSaV). Pairwise comparisons of protein sequences show that GarYSaV encodes proteins that share less than 47% identity with those of other foveaviruses, suggesting that it represents a new species in the genus. Phylogenetic analysis of amino acid sequences of the replicase and CP confirm that GarYSaV is a member of the genus Foveavirus. To our knowledge, this is the first report of a foveavirus in a monocot plant.
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