Temporal microRNA expression profile of pig peripheral blood during postnatal development
文献类型： 外文期刊
作者： Zhong, Zhijun 1 ; Zhu, Xingxing 2 ; Tang, Qianzi 2 ; Hong, Liang 3 ; Gu, Yiren 1 ; He, Zhiping 1 ; Tao, Xuan 1 ; Yang, Xu 1 ;
作者机构： 1.Sichuan Anim Sci Acad, Anim Breeding & Genet Key Lab Sichuan Prov, Chengdu 610066, Peoples R China
2.Sichuan Agr Univ, Coll Anim Sci & Technol, Inst Anim Genet & Breeding, Chengdu, Peoples R China
3.Sichuan Animtech Feed Corp Ltd, Chengdu, Peoples R China
4.Guizhou Acad Agr Sci, Inst Anim Husb & Vet Med, Guiyang, Peoples R China
关键词： Swine; whole blood; immunity; miRNA
期刊名称：ANIMAL BIOTECHNOLOGY （ 影响因子：2.282； 五年影响因子：1.702 ）
ISSN： 1049-5398
年卷期：
页码：
收录情况： SCI
摘要： Gene expression profiles of blood can reflect the physiopathologic status of the immune system. The dynamic microRNA (miRNA) expression profiles of peripheral blood from pigs at different developmental stages, and how differential expression of miRNAs might relate to immune system development, are unknown. In this study, peripheral blood samples taken at five developmental stages were used to construct 15 miRNA libraries (three biological replicates/stage): 0 days (newborn), 30 days (weaning), 60 days (weaned), and 180 and 360 days (puberty). We identified 295 known mature miRNAs. Hierarchical clustering of the miRNA expression profile showed significant differences between individuals at the neonatal and postnatal stages. Functional enrichment analysis revealed that miRNAs differentially expressed between pairwise comparisons of the developmental stages were over-represented in immune-related pathways such as toll-like receptor signaling. The time-course of expression of the over-representated miRNAs exhibited a pattern of steady decline over time, for both the complete miRNA compendium and immune-related miRNAs. We identified six marker miRNAs that were highly negatively correlated with chronologic age and enriched for genes involved in immune-related pathways. This study of a peripheral blood miRNA transcriptome offers insight into immune system development in swine and provides a resource for pig genome annotation.
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