Molecular characterization and expression analysis of double-stranded RNA-dependent protein kinase (PKR) in Dabry's sturgeon (Acipenser dabryanus)
文献类型： 外文期刊
作者： Guo, Huizhi 1 ; Chen, Dunxue 2 ; Yuan, Hanwen 1 ; Deng, Dan 1 ; Han, Panpan 1 ; Li, Qian 1 ; Xu, Qiaoqing 1 ; Wei, Qiwei 4 ;
作者机构： 1.Yangtze Univ, Sch Anim Sci, Jingzhou 434020, Peoples R China
2.Guizhou Univ, Res Ctr Fishery Resources & Environm, Guiyang, Peoples R China
3.Shantou Univ, Guangdong Prov Key Lab Marine Biotechnol, Shantou, Peoples R China
4.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Minist Agr, Key Lab Freshwater Biodivers Conservat, Wuhan, Peoples R China
关键词： Acipenser dabryanus; antiviral; expression; PKR; poly I; C stimulation
期刊名称：JOURNAL OF APPLIED ICHTHYOLOGY （ 影响因子：0.892； 五年影响因子：0.956 ）
ISSN： 0175-8659
年卷期： 2021 年 37 卷 2 期
页码：
收录情况： SCI
摘要： Protein kinase RNA-activated (PKR) is an important component of the innate immune system induced by interferon. This antiviral protein senses a variety of stresses and pathogen-driven double-stranded RNAs (dsRNAs). In the present study, we cloned and characterized the PKR gene from Dabry's sturgeon (Acipenser dabryanus). Bioinformatics analysis revealed that the AdPKR gene comprises a coding sequence (CDS) of 1848 bp, encoding a 616 amino acids (aa) polypeptide. AdPKR shares high aa identity/similarity with proteins from other fish, ranging from 33.4%/51.8% to 87.6%/91.2%, and relatively low aa identity (13.9%) and similarity (26.8%) with human PKR. The AdPKR polypeptide has two dsRNA-binding motifs at the N-terminus and a conserved catalytic kinase domain at the C-terminus. The sturgeon PKR gene contains 17 exons split by 16 introns. Phylogenetic analysis clustered AdPKR together with its counterparts from other teleost fishes. Real-time fluorescene quantitative PCR (RT-qPCR) transcription profiles revealed highest AdPKR expression levels in brain, followed by middle kidney, skin, muscle and head kidney. AdPKR mRNA transcripts were significantly increased at 6 hr post-stimulation (hps) with polyinosinic:polycytidylic acid (poly I:C), remained high at 24 hps, then decreased to normal levels at 48 hps. These results indicate that AdPKR might play a vital role in antiviral immune responses.
- 相关文献
作者其他论文 更多>>
-
Genetic diversity and population structure analysis of blunt snout bream (Megalobrama amblycephala) in the Yangtze River Basin: Implications for conservation and utilization
作者：Dong, Fang;Wei, Qiwei;Dong, Fang;Cheng, Peilin;Sha, Hang;Yue, Huamei;Wan, Chaoyang;Zhang, Yingyu;Zhang, Lang;Zhang, Hui;Wei, Qiwei;Wei, Qiwei
关键词：Blunt snout bream; Microsatellite; Mitochondrial DNA; Genetic diversity; Genetic differentiation
-
Sex-Dimorphic Differential Expression Profiles in the Brain of the Adult Chinese Soft-Shelled Turtle, Pelodiscus sinensis
作者：Liu, Pan;Xu, Qiaoqing;Zhou, Jiang;Liu, Pan;Liu, Yanchao;Zhu, Junxian;Chen, Chen;Ji, Liqin;Liu, Xiaoli;Hong, Xiaoyou;Wei, Chengqing;Zhu, Xinping;Li, Wei
关键词：
Pelodiscus sinensis ; RNA sequencing; sex-dimorphic growth; brain -
Structural Properties and Biological Activities of Collagens from Four Main Processing By-Products (Skin, Fin, Cartilage, Notochord) of Sturgeon (Acipenser gueldenstaedti)
作者：Meng, Dawei;Dai, Zhiyuan;Wei, Qiwei;Takagi, Yasuaki;Zhang, Yan
关键词：Sturgeon by-products; Collagen; Structural properties; Fibril-forming characteristics; Collagen peptides; Antioxidant activity
-
Pathogenicity and detection method based on interferon release assay of Mycobacteria marinum isolated from sturgeon
作者：Xie, Meng;Deng, Dan;Li, Qian;Zhang, Xiao;Luo, Kai;Zhou, Jiang;Xu, Qiaoqing;Li, Chenghua;Xu, Qiaoqing;Wang, Bei;Xu, Qiaoqing;Wei, Qiwei;Zhang, Shuhuan
关键词：Chinese sturgeon; Mycobacterium marinum; Antimicrobial-response; Disease detection
-
Extraction of chondroitin sulfate and type II collagen from sturgeon (Acipenser gueldenstaedti) notochord and characterization of their hybrid fibrils
作者：Meng, Dawei;Dai, Zhiyuan;Li, Wen;Leng, Xiaoqian;Du, Hao;Wei, Qiwei;Takagi, Yasuaki
关键词：Sturgeon Notochord Extraction Chondroitin sulfate; Type II collagen; Fibril
-
Intraperitoneal injection of 17 beta-estradiol increases ovarian smad2/3 expression in Yangtze sturgeon Acipenser dabryanus
作者：Yue, Huamei;Ye, Huan;Ruan, Rui;Du, Hao;Wei, Qiwei;Li, Chuangju
关键词：17 beta-estradiol; Acipenser dabryanus; ovary; Smad2; Smad3
-
Quantifying the Colonization of Environmental Microbes in the Fish Gut: A Case Study of Wild Fish Populations in the Yangtze River
作者：Yang, Haile;Wu, Jinming;Du, Hao;Zhang, Hui;Li, Junyi;Wei, Qiwei
关键词：gut microbiota; wild fish population; natural water; Yangtze River; environmental microbiota
