Antifungal activity of liquiritin in Phytophthora capsici comprises not only membrane-damage-mediated autophagy, apoptosis, and Ca2+ reduction but also an induced defense responses in pepper
文献类型： 外文期刊
作者： Liu, Peiqing 1 ; Cai, Yushan 1 ; Zhang, Jinzhu 1 ; Wang, Rongbo 1 ; Li, Benjin 1 ; Weng, Qiyong 1 ; Chen, Qinghe 1 ;
作者机构： 1.Fujian Acad Agr Sci, Inst Plant Protect, Fujian Key Lab Monitoring & Integrated Management, 247 Wusi Rd, Fuzhou 350003, Peoples R China
2.Hainan Univ, Coll Plant Protect, Key Lab Green Prevent & Control Trop Plant Dis &, Minist Educ, Haikou 570228, Hainan, Peoples R China
关键词： Phytophthora Capsici; Liquiritin; ROS; Membrane damage; Plant defense
期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：6.291； 五年影响因子：6.393 ）
ISSN： 0147-6513
年卷期： 2021 年 209 卷
页码：
收录情况： SCI
摘要： Phytophthora capsici causes a severe soil-borne disease in a wide variety of vegetables; to date, no effective strategies to control P. capsici have been developed. Liquiritin (LQ) is a natural flavonoid found in licorice (Glycyrrhiza spp.) root, and it is used in pharmaceuticals. However, the antifungal activity of LQ against P. capsici remains unknown. In the present study, we demonstrated that LQ inhibits P. capsici mycelial growth and sporangial development. In addition, the EC50 of LQ was 658.4 mg/L and LQ caused P. capsici sporangia to shrink and collapse. Next, LQ severely damaged the cell membrane integrity, leading to a 2.0-2.5-fold increase in relative electrical conductivity and malondialdehyde concentration, and a 65-70% decrease in sugar content. Additionally, the H2O2 content was increased about 2.0-2.5 fold, but the total antioxidant activity, catalase activity and laccase activity were attenuated by 40-45%, 30-35% and 70-75%. LQ also induced autophagy, apoptosis, and reduction of intracellular Ca2+ content. Furthermore, LQ inhibited P. capsici pathogenicity by reducing the expression of virulence genes PcCRN4 and Pc76RTF, and stimulating the plant defense (including the activated transcriptional expression of defense-related genes CaPR1, CaDEF1, and CaSAR82, and the increased antioxidant enzyme activity). Our results not only elucidate the antifungal mechanism of LQ but also suggest a promising alternative to commercial fungicides or a key compound in the development of new fungicides for the control of the Phytophthora disease.
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