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The complete mitochondrial genome of the hybrid grouper Epinephelus moara (female)xEpinephelus tukula (male), and phylogenetic analysis in subfamily Epinephelinae

文献类型： 外文期刊

作者： Li, Zhentong 1 ; Tian, Yongsheng 2 ; Cheng, Meiling 3 ; Wang, Linna 2 ; Zhang, Jingjing 1 ; Wu, Yuping 3 ; Pang, Zunfa 1 ;

作者机构： 1.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China

3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

4.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China

5.Laizhou Mingbo Aquat Co Ltd, Laizhou 261400, Peoples R China

关键词： Epinephelus moara (♀ )× Epinephelus tukula (♀ ); Epinephelus moara; Epinephelus tukula; mitochondrial genome; phylogeny

期刊名称：ACTA OCEANOLOGICA SINICA （ 影响因子：1.431； 五年影响因子：1.445 ）

ISSN： 0253-505X

年卷期： 2020 年 39 卷 12 期

页码：

收录情况： SCI

摘要： The mitochondrial genome (mitogenome) of hybrid grouper Epinephelus moara (female)xEpinephelus tukula (male), a new hybrid progeny, can provide valuable information for analyzing phylogeny and molecular evolution. In this study, the mitogenome was analyzed using PCR amplification and sequenced, then the phylogenetic relationship of E. moara (female)xE. tukula (male) and 35 other species were constructed using Maximum Likelihood and Neighbor-Joining methods with the nucleotide sequences of 13 conserved protein-coding genes (PCGs). The complete mitogenome of E. moara (female)xE. tukula (male) was 16 695 bp in length, which contained 13 PCGs, 2 rRNA genes, 22 tRNA genes, a replication origin and a control region. The composition and order of these genes were consistent with most other vertebrates. Of the 13 PCGs, 12 PCGs were encoded on the heavy strand, and ND6 was encoded on the light strand. The mitogenome of the E. moara (female)xE. tukula (male) had a higher AT nucleotide content, a positive AT-skew and a negative GC-skew. All protein initiation codons were ATG, except for COX and ND4 (GTG), ATP6 (CTG), and ND3 (ATA). ND2, COXII, ND3, ND4 and Cytb had T as the terminating codon, COXIII's termination codon was TA, and the remaining PCGs of that were TAA. All tRNA genes, except for the lacking DHU-arm of tRNA(Ser)((AGN)), were predicted to form a typical cloverleaf secondary structure. In addition, sequence similarity analysis (99% identity) and phylogenetic analysis (100% bootstrap value) indicated that the mitochondrial genome was maternally inherited. This study provides mitogenome data for studying genetic, phylogenetic relationships and breeding of grouper.

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