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The Arabidopsis sucrose non-fermenting-1-related protein kinase AtSnRK2.4 interacts with a transcription factor, AtMYB21, that is involved in salt tolerance

文献类型： 外文期刊

作者： Zhang, Xinxin 1 ; Wu, Shan 4 ; Liu, Shenkui 6 ; Takano, Tetsuo 7 ;

作者机构： 1.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Guangzhou 510640, Peoples R China

2.Key Lab South Subtrop Fruit Biol & Genet Resource, Guangzhou 510640, Peoples R China

3.Guangdong Prov Key Lab Trop & Subtrop Fruit Tree, Guangzhou 510640, Peoples R China

4.Minist Educ, Key Lab Saline Alkali Vegetat Ecol Restorat SAVER, Harbin 150040, Peoples R China

5.Northeast Forestry Univ, Alkali Soil Nat Environm Sci Ctr ASNESC, Harbin 150040, Peoples R China

6.Zhejiang A&F Univ, State Key Lab Subtrop Silviculture, Hangzhou 311300, Peoples R China

7.Univ Tokyo, Asian Nat Environm Sci Ctr ANESC, 1-1-1 Midori Cho, Nishitokyo, Tokyo 1880002, Japan

关键词： Arabidopsis; AtSnRK2.4; AtMYB21; Salt stress

期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）

ISSN： 0168-9452

年卷期： 2021 年 303 卷

页码：

收录情况： SCI

摘要： Sucrose non-fermenting-1-related protein kinase 2 s (SnRK2 s) are important stress-related plant protein kinases in plants. The interaction partners and phosphorylation substrates of group II and III SnRK2 s in Arabidopsis thaliana have been identified, but similar data for group I SnRK2 s are very limited. Here, we used a yeast two-hybrid (Y2H) screen to find proteins that interact with Arabidopsis AtSnRK2.4, a group I SnRK2. The transcription factor AtMYB21 was identified as an AtSnRK2.4 interaction partner, and its interaction with AtSnRK2.4 was confirmed by an in vitro pull-down assay and a bimolecular fluorescence complementation (BiFC) assay. A subcellular localization assay demonstrated that AtSnRK2.4 and AtMYB21 were located in the cytoplasm and nucleus of onion epidermal cells. AtSnRK2.4 and AtMYB21 were expressed in many tissues and upregulated in response to NaCl stress. Transgenic plants that overexpressed AtSnRK2.4 or AtMYB21 gene exhibited enhanced tolerance to salt stress at germination and post-germination stages. Moreover, the expression of downstream stress-responsive genes was upregulated in salt-stressed AtSnRK2.4 and AtMYB21 transgenic Arabidopsis. These results suggest that AtSnRK2.4 may act synergistically with AtMYB21 to mediate the response to salt stress through the upregulation of downstream stress-responsive genes.

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