Molecular cloning, characterization and functional analysis of two neuropeptide F genes from the oriental river prawn (Macrobrachium nipponense)
文献类型： 外文期刊
作者： Qiao, Hui 1 ; Jiang, Sufei 1 ; Xiong, Yiwei 1 ; Zhang, Wenyi 1 ; Xu, Lei 2 ; Jin, Shubo 1 ; Gong, Yongsheng 1 ; Wu, Yan 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resource, Minist Agr, Wuxi, Jiangsu, Peoples R China
2.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi, Jiangsu, Peoples R China
关键词： Macrobrachium nipponense; Neuropeptide F; Ovary maturation; mRNA expression; RNA interference
期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY （ 影响因子：2.32； 五年影响因子：2.465 ）
ISSN： 1095-6433
年卷期： 2021 年 253 卷
页码：
收录情况： SCI
摘要： In invertebrates, neuropeptide F (NPF) has many regulatory functions, similar to NPY, its homologous peptide. In this study, two neuropeptide F genes were identified in Macrobrachium nipponense: Mn-NPF1 and Mn-NPF2. Mn-NPF2 shared the same amino acid sequence with Mn-NPF1, except for a 37 amino acid insert in the middle of the NPF region. The quantitative-PCR (qPCR) results indicated that Mn-NPF1 expression was positively correlated with ovarian maturation, whereas Mn-NPF2 had opposing expression patterns. Both Mn-NPFs were poorly expressed at early embryonic stages, but enhanced expression levels were observed up to day 10 after hatching, when the gonads began to differentiate. Ovary in situ hybridization (ISH) analyses showed that both Mn-NPFs were present at all stages, but were differentially localized to distinct compartments. Temperature gradient studies showed that both Mn-NPFs were implicated in the seasonal regulation of reproduction. A double-stranded (ds) RNA-Mn-NPF2 injection led to a significant 38.5% increase in the vitellogenin (VG) transcript (P < 0.05). These results demonstrated that Mn-NPF2 plays an important role in inhibiting ovarian maturation.
- 相关文献
作者其他论文 更多>>
-
Characterization of the Complete Mitochondrial Genome of Dwarf Form of Purpleback Flying Squid (Sthenoteuthis oualaniensis) and Phylogenetic Analysis of the Family Ommastrephidae
作者：Duo, Wenjuan;Yusof, Mohd Johari Mohd;Xu, Lei;Du, Feiyan;Xu, Lei;Wang, Yingmin;Ng, Seng Beng
关键词：Ommastrephidae; mitochondrial genome; phylogenetic;
Sthenoteuthis ; dwarf form -
Population genetic structure and historical demography of Saccostrea echinata in the Northern South China sea and Beibu Gulf
作者：Li, Yafang;Wang, Lianggen;Tang, Quehui;Liu, Shuangshuang;Ning, Jiajia;Huang, Delian;Xu, Lei;Du, Feiyan;Li, Yafang;Wang, Lianggen;Tang, Quehui;Liu, Shuangshuang;Ning, Jiajia;Huang, Delian;Xu, Lei;Du, Feiyan;Li, Yafang;Wang, Lianggen;Tang, Quehui;Liu, Shuangshuang;Ning, Jiajia;Huang, Delian;Xu, Lei;Du, Feiyan;Wang, Yingmin
关键词：Marine invertebrates; Genetic differentiation; Black-lip oyster (Saccostrea echinata); Mitochondrial DNA (COI gene); Population structure; Historical Climatic events
-
The diversity and biogeography of macrobenthic fauna in the rocky intertidal zone of islands in the Pearl River estuary: effects of island area, isolation, salinity and human disturbance
作者：Li, Yafang;Tang, Quehui;Liu, Shuangshuang;Wang, Lianggen;Ning, Jiajia;Xu, Lei;Huang, Delian;Du, Feiyan;Li, Yafang;Tang, Quehui;Liu, Shuangshuang;Wang, Lianggen;Ning, Jiajia;Xu, Lei;Huang, Delian;Du, Feiyan
关键词：Species-area relationship; Beta diversity; Heterogeneous; Taxonomic groups; Spatial isolation; Salinity
-
Identification of potential roles of cathepsin L1 in the response to alkaline treatment in Macrobrachium nipponense
作者：Xu, Mingjia;Fu, Hongtuo;Qiao, Hui;Jiang, Sufei;Jin, Shubo;Zhang, Wenyi;Xiong, Yiwei;Fu, Hongtuo;Qiao, Hui;Jiang, Sufei;Jin, Shubo
关键词：
Macrobrachium nipponense ; Alkaline treatment; Cathepsin L1; QPCR; RNAi -
Functional Characterization of Two β-Hexosaminidase A Isoforms During Ovarian Development in Macrobrachium nipponense
作者：Wang, Zhiming;Jiang, Sufei;Fu, Hongtuo;Wang, Zhiming;Jiang, Sufei;Zhang, Wenyi;Jin, Shubo;Xiong, Yiwei;Qiao, Hui;Fu, Hongtuo;Jiang, Sufei;Jin, Shubo;Xu, Ming;Gao, Zijian;Xu, Mingjia;Qiao, Hui;Fu, Hongtuo
关键词：
Macrobrachium nipponense ;beta-hexosaminidase A ; ovarian maturation; RNAi -
Evaluation and Comparison Germplasm Resource of Two Species of Macrobrachium in China (M. hainanense and M. nipponense)
作者：Eddine, Khadraoui Djamel;Gao, Zijian;Daka, Peter;Fu, Hongtuo;Zhang, Wenyi;Jiang, Sufei;Xiong, Yiwei;Qiao, Hui;Fu, Hongtuo
关键词：
-
Investigation of the Effects of Salinity Exposure on Immune Defense, Morphology, and Gene Expression in the Gills of Macrobrachium nipponense
作者：Jin, Shubo;Zhou, Rong;Fu, Hongtuo;Qiao, Hui;Jiang, Sufei;Jin, Shubo;Fu, Hongtuo;Zhang, Wenyi;Qiao, Hui;Xiong, Yiwei;Jiang, Sufei
关键词：Macrobrachium nipponense; salinity; gill; immune response; gene response
