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A label-free aptasensor for turn-on fluorescent detection of ochratoxin a based on SYBR gold and single walled carbon nanohorns

文献类型： 外文期刊

作者： Guo, Zhijun 2 ; Tian, Jing 2 ; Cui, Chengbi 2 ; Wang, Yan 3 ; Yang, Huanhuan 4 ; Yuan, Ming 6 ; Yu, Hansong 1 ;

作者机构： 1.Jilin Agr Univ, Coll Food Sci & Engn, Changchun 130118, Peoples R China

2.Yanbian Univ, Coll Agr, Yanji 133002, Peoples R China

3.Zhejiang Agr & Forestry Univ, Coll Agr & Food Sci, Hangzhou 310000, Peoples R China

4.Heilongjiang Bayi Agr Univ, Coll Food Sci, Daqing 163000, Peoples R China

5.Chinese Agr Res Syst, Soybean Res & Dev Ctr, Div Soybean Proc, Changchun 130118, Peoples R China

6.Heilongjiang Acad Agr Sci, Qiqihar Branch, Qiqihar 161006, Peoples R China

关键词： Ochratoxin a; Label-free; Aptasensor; SYBR Gold; Single walled carbon nanohorns

期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）

ISSN： 0956-7135

年卷期： 2021 年 123 卷

页码：

收录情况： SCI

摘要： Ochratoxin A (OTA), is one of the most commonly found mycotoxins in food. It is primarily produced by Aspergillus ochraceus and Penicillium verrucosum. OTA can causes serious damages to liver and kidney, increases the teratogenicity, and has been considered as a potential carcinogen (group 2 B). In this study, we demonstrated a new detection system for the detection of OTA using fluorescence biosensing according to an anti-OTA aptamer and fluorescence quencher using single-walled carbon nanohorns (SWCNHs). In the presence of OTA, the OTA-specific aptamer underwent the conformation change from a random coil to an antiparallel G-quadruplex. The latter one was resistant to being adsorbed onto SWCNHs, leading to the pertection of the fluorescence of SYBR Gold. As a result, the remained fluorescent intensities correlated with the concentrations of OTA. The detection limit of OTA was 2.3 ng/ml, meanwhile the linear detection range was 5-500 ng/ml, with a satisfactory linear correlation (R-2 = 0.992) of the fluorescence intensity and the logarithm of OTA concentrations. The aptasensor was subjected to quantify the concentration of OTA in red wine samples. As a result, this label-free aptasensor was suitable for the test of OTA in food samples.

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