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Overexpression of ZmWRKY65 transcription factor from maize confers stress resistances in transgenic Arabidopsis

文献类型： 外文期刊

作者： Huo, Tong 1 ; Wang, Chang-Tao 1 ; Yu, Tai-Fei 2 ; Wang, Da-Ming 3 ; Li, Meng 1 ; Zhao, Dan 1 ; Li, Xiu-Ting 1 ; Fu, Jin-D 1 ;

作者机构： 1.Beijing Technol & Business Univ, Inst Cosmet Regulatory Sci, Beijing Key Lab Plant Resource Res & Dev, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100048, Peoples R China

2.Chinese Acad Agr Sci CAAS, Natl Key Facil Crop Gene Resources & Genet Improv, Key Lab Biol & Genet Improvement Triticeae Crops, Inst Crop Sci,Minist Agr, Beijing 100081, Peoples R China

3.Jilin Acad Agr Sci, Agrobiotechnol Res Inst, Changchun 130033, Peoples R China

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2021 年 11 卷 1 期

页码：

收录情况： SCI

摘要： Plant-specific WRKY transcription factors play important roles in regulating the expression of defense-responsive genes against pathogen attack. A multiple stress-responsive WRKY gene, ZmWRKY65, was identified in maize by screening salicylic acid (SA)-induced de novo transcriptomic sequences. The ZmWRKY65 protein was localized in the nucleus of mesophyll protoplasts. The analysis of the ZmWRKY65 promoter sequence indicated that it contains several stress-related transcriptional regulatory elements. Many environmental factors affecting the transcription of ZmWRKY65 gene, such as drought, salinity, high temperature and low temperature stress. Moreover, the transcription of ZmWRKY65 gene was also affected by the induction of defense related plant hormones such as SA and exogenous ABA. The results of seed germination and stomatal aperture assays indicated that transgenic Arabidopsis plants exhibit enhanced sensitivity to ABA and high concentrations of SA. Overexpression of ZmWRKY65 improved tolerance to both pathogen attack and abiotic stress in transgenic Arabidopsis plants and activated several stress-related genes such as RD29A, ERD10, and STZ as well as pathogenesis-related (PR) genes such as PR1, PR2 and PR5; these genes are involved in resistance to abiotic and biotic stresses in Arabidopsis. Together, this evidence implies that the ZmWRKY65 gene is involved in multiple stress signal transduction pathways.

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