文献类型： 外文期刊

作者： Yu, Tai-Fei ¹ ; Hou, Ze-Hao ¹ ; Wang, Hai-Long ² ; Chang, Shi-Yang ³ ; Song, Xin-Yuan ⁴ ; Zheng, Wei-Jun ⁵ ; Zheng, Lei ¹ ; Wei, Ji-Tong ¹ ; Lu, Zhi-Wei ¹ ; Chen, Jun ¹ ; Zhou, Yong-Bin ¹ ; Chen, Ming ¹ ; Sun, Su-Li ¹ ; Jiang, Qi-Yan ¹ ; Jin, Long-Guo ¹ ; Ma, You-Zhi ¹ ; Xu, Zhao-Shi ¹ ;

作者机构： 1.Chinese Acad Agr Sci CAAS, Inst Crop Sci, State Key Lab Crop Gene Resources & Breeding, Beijing, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Biotechnol, Beijing Key Lab Agr Genet Resources & Biotechnol, Beijing, Peoples R China

3.Hebei Med Univ, Dept Histol & Embryol, Shijiazhuang, Hebei, Peoples R China

4.Jilin Acad Agr Sci, Agrobiotechnol Res Inst, Changchun, Peoples R China

5.Northwest Agr & Forestry Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling, Peoples R China

6.Jilin Agr Univ, Coll Agron, Coll Life Sci, Changchun, Peoples R China

7.Chinese Acad Agr Sci, Natl Nanfan Res Inst Sanya, Seed Ind Lab, Sanya, Peoples R China

关键词： soybean (Glycine max); plant steroid hormone; abiotic stress; regulated mechanism; squalene monooxygenase

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：13.8； 五年影响因子：13.2 ）

ISSN： 1467-7644

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Sterols have long been associated with diverse fields, such as cancer treatment, drug development, and plant growth; however, their underlying mechanisms and functions remain enigmatic. Here, we unveil a critical role played by a GmNF-YC9-mediated CCAAT-box transcription complex in modulating the steroid metabolism pathway within soybeans. Specifically, this complex directly activates squalene monooxygenase (GmSQE1), which is a rate-limiting enzyme in steroid synthesis. Our findings demonstrate that overexpression of either GmNF-YC9 or GmSQE1 significantly enhances soybean stress tolerance, while the inhibition of SQE weakens this tolerance. Field experiments conducted over two seasons further reveal increased yields per plant in both GmNF-YC9 and GmSQE1 overexpressing plants under drought stress conditions. This enhanced stress tolerance is attributed to the reduction of abiotic stress-induced cell oxidative damage. Transcriptome and metabolome analyses shed light on the upregulation of multiple sterol compounds, including fucosterol and soyasaponin II, in GmNF-YC9 and GmSQE1 overexpressing soybean plants under stress conditions. Intriguingly, the application of soybean steroids, including fucosterol and soyasaponin II, significantly improves drought tolerance in soybean, wheat, foxtail millet, and maize. These findings underscore the pivotal role of soybean steroids in countering oxidative stress in plants and offer a new research strategy for enhancing crop stress tolerance and quality from gene regulation to chemical intervention.