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A salivary calcium-binding protein from Laodelphax striatellus acts as an effector that suppresses defense in rice

文献类型： 外文期刊

作者： Tian, Tian 1 ; Ji, Rui 2 ; Fu, Jianmei 2 ; Li, Jing 2 ; Wang, Lu 1 ; Zhang, Hao 1 ; Yang, Shiying 1 ; Ye, Wenfeng 3 ; Fang, 1 ;

作者机构： 1.Nanjing Agr Univ, Dept Entomol, Nanjing 210014, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Minist Sci & Technol, Inst Plant Protect, Jiangsu Key Lab Food & Safety,State Key Lab Culti, Nanjing, Peoples R China

3.Univ Neuchatel, Lab Fundamental & Appl Res Chem Ecol, Neuchatel, Switzerland

4.Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA

关键词： effector; EF‐ hand Ca2+‐ binding protein; plant– insect interaction; plant‐ mediated RNAi; small brown planthopper

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）

ISSN： 1526-498X

年卷期： 2021 年 77 卷 5 期

页码：

收录情况： SCI

摘要： BACKGROUND Calcium (Ca2+)-binding proteins in the saliva of herbivorous insects function as effectors to attenuate host plant defenses and thus improve insect feeding performance. Silencing these genes via transgenic plant-mediated RNAi is thus a promising pest control strategy. However, their sequences and functions in the small brown planthopper Laodelphax striatellus (SBPH) remain to be investigated. RESULTS We identified a putative EF-hand Ca2+-binding protein (LsECP1) in SBPH watery saliva. LsECP1 was expressed extremely high in the salivary glands but at a low level during the egg stage. Transient LsECP1 expression in rice cells indicated its cytoplasm and nucleus localization. The bacterially expressed recombinant LsECP1 protein exhibited Ca2+-binding activity. Rice plants fed by SBPH nymphs with knocked down LsECP1 exhibited higher levels of cytosolic Ca2+, jasmonic acid (JA), jasmonoyl-isoleucine (JA-Ile) and hydrogen peroxide (H2O2). Consistently, application of heterogeneously expressed LsECP1 protein suppressed wound-induced JA, JA-Ile and H2O2 accumulation in rice. Thus, LsECP1 knockdown by dsRNA injection resulted in reduced feeding, fecundity and survival rates of SBPH reared on rice plants. Transgenic rice plants constitutively expressing LsECP1 dsRNA were produced, and plant-mediated LsECP1 knockdown enhanced rice resistance to SBPH. CONCLUSION SBPH LsECP1 acts as an effector to impair host rice defense responses and promotes SBPH performance. This discovery provides a potential gene target for plant-mediated RNAi-based pest management.

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