Integrating Transcriptomics and Metabolomics to Characterize Metabolic Regulation to Elevated CO2 in Chlamydomonas Reinhardtii
文献类型： 外文期刊
作者： Zhang, Yufei 1 ; Gu, Zipeng 1 ; Ren, Yudong 1 ; Wang, Lu 1 ; Zhang, Jian 1 ; Liang, Chengwei 1 ; Tong, Shanying 2 ; Wang, 1 ;
作者机构： 1.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, 53 Zhengzhou Rd, Qingdao 266042, Peoples R China
2.Ludong Univ, Sch Life Sci, 186 Hongqi Middle Rd, Yantai 264025, Peoples R China
3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, 106 Nanjing Rd, Qingdao 266071, Peoples R China
4.Natl Oceanog Ctr, Qingdao 266071, Peoples R China
关键词： Chlamydomonas Reinhardtii; Elevated CO2; Long-term Acidification; Metabolomics; Transcriptomics
期刊名称：MARINE BIOTECHNOLOGY （ 影响因子：3.619； 五年影响因子：3.322 ）
ISSN： 1436-2228
年卷期： 2021 年 23 卷 2 期
页码：
收录情况： SCI
摘要： With atmospheric CO2 increasing, a large amount of CO2 is absorbed by oceans and lakes, which changes the carbonate system and affects the survival of aquatic plants, especially microalgae. The main aim of our study was to explore the responses of Chlamydomonas reinhardtii (Chlorophyceae) to elevated CO2 by combined transcriptome and metabolome analysis under three different scenarios: control (CK, 400 ppm), short-term elevated CO2 (ST, 1000 ppm), and long-term elevated CO2 (LT, 1000 ppm). The transcriptomic data showed moderate changes between ST and CK. However, metabolic analysis indicated that fatty acids (FAs) and partial amino acids (AAs) were increased under ST. There was a global downregulation of genes involved in photosynthesis, glycolysis, lipid metabolism, and nitrogen metabolism but increase in the TCA cycle and beta-oxidation under LT. Integrated transcriptome and metabolome analyses demonstrated that the nutritional constituents (FAs, AAs) under LT were poor compared with CK, and most genes and metabolites involved in C and N metabolism were significantly downregulated. However, the growth and photosynthesis of cells under LT increased significantly. Thus, C. reinhardtii could form a specific adaptive evolution to elevated CO2, affecting future biogeochemical cycles.
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