您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Transcriptomes analysis reveals novel insight into the molecular mechanisms of somatic embryogenesis in Hevea brasiliensis

文献类型： 外文期刊

作者： Wang, Ying 1 ; Li, Hui-Liang 1 ; Zhou, Yong-Kai 1 ; Guo, Dong 1 ; Zhu, Jia-Hong 1 ; Peng, Shi-Qing 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Minist Agr, Inst Trop Bioscience & Biotechnol, Key Lab Biol & Genet Resources Trop Crops, 4 Xueyuan Rd, Haikou 571101, Hainan, Peoples R China

2.Hainan Univ, Sch Life & Pharmaceut Sci, Haikou 570228, Hainan, Peoples R China

3.CATAS, Hainan Acad Trop Agr Resource, Haikou 571101, Hainan, Peoples R China

关键词： Hevea brasiliensis; Somatic embryogenesis; RNA-seq; Hormone signal; Transcription factor; Histone modification

期刊名称：BMC GENOMICS （ 影响因子：3.594； 五年影响因子：4.093 ）

ISSN： 1471-2164

年卷期： 2021 年 22 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundSomatic embryogenesis (SE) is a promising technology for plant vegetative propagation, which has an important role in tree breeding. Though rubber tree (Hevea brasiliensis Muell. Arg.) SE has been founded, few late SE-related genes have been identified and the molecular regulation mechanisms of late SE are still not well understood.ResultsIn this study, the transcriptomes of embryogenic callus (EC), primary embryo (PE), cotyledonary embryo (CE), abnormal embryo (AE), mature cotyledonary embryo (MCE) and withered abnormal embryo (WAE) were analyzed. A total of 887,852,416 clean reads were generated, 85.92% of them were mapped to the rubber tree genome. The de novo assembly generated 36,937 unigenes. The differentially expressed genes (DEGs) were identified in the pairwise comparisons of CE vs. AE and MCE vs. WAE, respectively. The specific common DEGs were mainly involved in the phytohormones signaling pathway, biosynthesis of phenylpropanoid and starch and sucrose metabolism. Among them, hormone signal transduction related genes were significantly enriched, especially the auxin signaling factors (AUX-like1, GH3.1, SAUR32-like, IAA9-like, IAA14-like, IAA27-like, IAA28-like and ARF5-like). The transcription factors including WRKY40, WRKY70, MYBS3-like, MYB1R1-like, AIL6 and bHLH93-like were characterized as molecular markers for rubber tree late SE. CML13, CML36, CAM-7, SERK1 and LEAD-29-like were also related to rubber tree late SE. In addition, histone modification had crucial roles during rubber tree late SE.ConclusionsThis study provides important information to elucidate the molecular regulation during rubber tree late SE.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台