Overproduction of alpha-Farnesene in Saccharomyces cerevisiae by Farnesene Synthase Screening and Metabolic Engineering
文献类型： 外文期刊
作者： Wang, Junhua 1 ; Jiang, Wei 1 ; Liang, Chaojuan 2 ; Zhu, Linghuan 1 ; Li, Youran 1 ; Mo, Qin 4 ; Xu, Sha 1 ; Chu, Alex 1 ; Z 1 ;
作者机构： 1.Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Jiangsu, Peoples R China
2.Jiangnan Univ, Natl Engn Lab Cereal Fermentat Technol, Wuxi 214122, Jiangsu, Peoples R China
3.Jiangnan Univ, Jiangsu Prov Res Ctr Bioact Prod Proc Technol, Wuxi 214122, Jiangsu, Peoples R China
4.Shanghai Acad Agr Sci, Biotechnol Res Inst, Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China
关键词： alpha-farnesene; farnesene synthase; HMG-CoA reductase; squalene; metabolic engineering; Saccharomyces cerevisiae
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：4.192； 五年影响因子：4.289 ）
ISSN： 0021-8561
年卷期： 2021 年 69 卷 10 期
页码：
收录情况： SCI
摘要： Maximizing the flux of farnesyl diphosphate (FPP) to farnesene biosynthesis is the main challenge of farnesene overproduction in Saccharomyces cerevisiae. In this study, we screened a-farnesene synthase from soybean (Fsso) with a higher catalytic ability. Combining the overexpression of the mevalonate (MVA) pathway with the expression of Fsso, an engineered yeast strain producing 190.5 mg/L alpha-farnesene was screened with poor growth. By decreasing the copies of 3-hydroxy-3-methylglutarylcoenzyme (HMGR) overexpressed, the titer was increased to 417.8 mg/L. Then, the coexpression of Fsso and HMGR under the control of the GAL promoter and inactivation of lipid phosphate phosphatase encoded by DPP1 promoted the titer to 1163.7 mg/L. The titer was further increased to 1477.2 mg/L at the shake flask level with better growth by the construction of a prototrophic strain. Finally, the highest alpha-farnesene production of 10.4 g/L in S. cerevisiae was obtained by fed-batch fermentation in a 5 L bioreactor.
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