Overproduction of alpha-Farnesene in Saccharomyces cerevisiae by Farnesene Synthase Screening and Metabolic Engineering
文献类型： 外文期刊
作者： Wang, Junhua 1 ; Jiang, Wei 1 ; Liang, Chaojuan 2 ; Zhu, Linghuan 1 ; Li, Youran 1 ; Mo, Qin 4 ; Xu, Sha 1 ; Chu, Alex 1 ; Z 1 ;
作者机构： 1.Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Jiangsu, Peoples R China
2.Jiangnan Univ, Natl Engn Lab Cereal Fermentat Technol, Wuxi 214122, Jiangsu, Peoples R China
3.Jiangnan Univ, Jiangsu Prov Res Ctr Bioact Prod Proc Technol, Wuxi 214122, Jiangsu, Peoples R China
4.Shanghai Acad Agr Sci, Biotechnol Res Inst, Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China
关键词： alpha-farnesene; farnesene synthase; HMG-CoA reductase; squalene; metabolic engineering; Saccharomyces cerevisiae
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：4.192； 五年影响因子：4.289 ）
ISSN： 0021-8561
年卷期： 2021 年 69 卷 10 期
页码：
收录情况： SCI
摘要： Maximizing the flux of farnesyl diphosphate (FPP) to farnesene biosynthesis is the main challenge of farnesene overproduction in Saccharomyces cerevisiae. In this study, we screened a-farnesene synthase from soybean (Fsso) with a higher catalytic ability. Combining the overexpression of the mevalonate (MVA) pathway with the expression of Fsso, an engineered yeast strain producing 190.5 mg/L alpha-farnesene was screened with poor growth. By decreasing the copies of 3-hydroxy-3-methylglutarylcoenzyme (HMGR) overexpressed, the titer was increased to 417.8 mg/L. Then, the coexpression of Fsso and HMGR under the control of the GAL promoter and inactivation of lipid phosphate phosphatase encoded by DPP1 promoted the titer to 1163.7 mg/L. The titer was further increased to 1477.2 mg/L at the shake flask level with better growth by the construction of a prototrophic strain. Finally, the highest alpha-farnesene production of 10.4 g/L in S. cerevisiae was obtained by fed-batch fermentation in a 5 L bioreactor.
- 相关文献
作者其他论文 更多>>
-
A dual-RPA based lateral flow strip for sensitive, on-site detection of CP4-EPSPS and Cry1Ab/Ac genes in genetically modified crops
作者：Wang, Jinbin;Wang, Yu;Hu, Xiuwen;Chen, Yifan;Jiang, Wei;Zeng, Haijuan;Liu, Hua;Wang, Jinbin;Chen, Yifan;Jiang, Wei;Zeng, Haijuan;Liu, Hua;Wang, Yu;Liu, Xiaofeng;Liu, Juan;Zhu, Lemei;Liu, Juan;Zhu, Lemei;Zeng, Haijuan;Liu, Hua
关键词：Genetically modified crops; On-site detection; Lateral flow test strips; Dual recombinase polymerase amplification; (RPA)
-
Plant growth-promoting rhizobacteria Bacillus velezensis JB0319 promotes lettuce growth under salt stress by modulating plant physiology and changing the rhizosphere bacterial community
作者：Bai, Yinshuang;Zhou, Yifan;Huang, Yanna;He, Chuan;Jiang, Wei;Liu, Hua;Zeng, Haijuan;Wang, Jinbin;He, Chuan;Jiang, Wei;Liu, Hua;Zeng, Haijuan;Wang, Jinbin;Bai, Yinshuang;Yue, Tong
关键词：Salt stress; Plant growth -promoting rhizobacteria; Lettuce; Active oxygen scavenging; Osmotic adjustment; Bacterial community
-
Biocontrol of tomato bacterial wilt by a combination of Bacillus subtilis GSJB-1210 and ningnanmycin
作者：Wang, Jinbin;He, Chuan;Zhou, Yifan;Bai, Yinshuang;Li, Qingwei;Jiang, Wei;Huang, Yanna;He, Chuan;Jiang, Wei;Wang, Jinbin;Yue, Tong;Liu, Xiaofeng
关键词：Tomato bacterial wilt; Bactericides; Bacillus subtilis; Ningnanmycin; Combined treatment
-
Engineering Saccharomyces cerevisiae YPH499 for Overproduction of Geranylgeraniol
作者：Wang, Junhua;Wang, Junhua;Chen, Wei;Li, Youran;Jiang, Wei;Gu, Zhenghua;Xu, Sha;Zhang, Liang;Ding, Zhongyang;Shi, Guiyang;Li, Youran;Jiang, Wei;Gu, Zhenghua;Xu, Sha;Zhang, Liang;Ding, Zhongyang;Shi, Guiyang;Jiang, Wei;Hu, Jinyuan
关键词：geranylgeraniol; squalene; geranylgeranyl diphosphatesynthase; mevalonate pathway; Saccharomycescerevisiae
-
A Rapid and Visual Method for Nucleic Acid Detection of Escherichia coli O157:H7 Based on CRISPR/Cas12a-PMNT
作者：Jiang, Wei;He, Chuan;Bai, Lan;Chen, Yifan;Jia, Junwei;Lv, Beibei;Tang, Xueming;Wu, Xiao;Jiang, Wei;He, Chuan;Bai, Lan;Chen, Yifan;Pan, Aihu;Lv, Beibei;Tang, Xueming;Wu, Xiao;Jiang, Wei;He, Chuan;Bai, Lan;Chen, Yifan;Jia, Junwei;Pan, Aihu;Lv, Beibei;Tang, Xueming;Wu, Xiao;Tang, Xueming
关键词：foodborne pathogenic bacteria; Escherichia coli O157; H7; nucleic acid detection; POCT; visual method; CRISPR-Cas12a; PMNT
-
Thermal, structural, rheological and morphological properties of potato starch-gluten model dough systems: Effect of degree of starch pre-gelatinization
作者：Xu, Fen;Liu, Wei;Zhang, Liang;Liu, Qiannan;Wang, Feng;Zhang, Hong;Hu, Honghai;Blecker, Christophe
关键词：Starch gelatinization; Gluten network; Dough; Rheology; Structural properties
-
Screening and production of dsRNA molecules for protecting Cucumis sativus against Cucumber mosaic virus through foliar application
作者：Mo, Qin;Lv, Beibei;Sun, Yu;Wu, Xiao;Song, Lili;Tang, Xueming;Cai, Run
关键词：Cucumber mosaic virus; Double-stranded RNA; RNA-mediated virus resistance; Crop protection
