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Screening and production of dsRNA molecules for protecting Cucumis sativus against Cucumber mosaic virus through foliar application

文献类型： 外文期刊

作者： Mo, Qin 1 ; Lv, Beibei 1 ; Sun, Yu 1 ; Wu, Xiao 1 ; Song, Lili 1 ; Cai, Run 2 ; Tang, Xueming 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Biotechnol Res Inst, Key Lab Agr Genet & Breeding, 2901 Bei Di Rd, Shanghai 201106, Peoples R China

2.Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai 200240, Peoples R China

关键词： Cucumber mosaic virus; Double-stranded RNA; RNA-mediated virus resistance; Crop protection

期刊名称：PLANT BIOTECHNOLOGY REPORTS （ 影响因子：2.496； 五年影响因子：2.406 ）

ISSN： 1863-5466

年卷期： 2022 年 16 卷 4 期

页码：

收录情况： SCI

摘要： Cucumber mosaic virus (CMV), which is one of the most prevalent plant viruses, harms many crops around the word. Although CMV causes severe economic losses to agriculture, successful prevention and treatment strategies are lacking. RNA interference (RNAi) is a promising strategy for the control of CMV because of its impressive specificity and sustainability. Here, a bacterial expression approach was conducted to produce hairpin RNA, and the best expression conditions to produce a high yield of dsRNA were explored. The highest expression of dsRNA in Escherichia coli HT115 was achieved at 37 degrees C using 2 mM IPTG and 17 h post-induction. For resistance analysis, six dsRNA molecules were constructed for CMV 1a, 2a, 3a and coat protein (CP). In cucumber (Cucumis sativus), foliar application of dsRNA preparations derived from CMV-1a and CMV-2a proteins conferred efficient protection against CMV. Our findings confirm that the exogenous application of dsRNA with a bacterial prokaryotic expression system could be a powerful biotechnological tool to protect plants against virus infections.

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