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Transcriptomic insights into immune responses to ulcerative syndrome in & nbsp;Pseudobagrus ussuriensis

文献类型： 外文期刊

作者： Zhu, Chuankun 1 ; Pan, Zhengjun 1 ; Chang, Guoliang 1 ; Wu, Nan 1 ; Wang, Hui 1 ; Ding, Huaiyu 1 ; Qiang, Xiaogang 2 ; Zh 1 ;

作者机构： 1.Huaiyin Normal Univ, Jiangsu Collaborat Innovat Ctr Reg Modern Agr & E, Jiangsu Engn Lab Breeding Special Aquat Organisms, Huaian 223300, Peoples R China

2.Huaian Fisheries Tech Guidance Stn, Huaian 223001, Peoples R China

3.Chinese Acad Sci, Huaian Sub Ctr, Inst Hydrobiol, Key Lab Fishery Sustainable Dev & Water Environm, Huaian 223002, Peoples R China

4.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resource, Minist Agr, Wuxi 214081, Jiangsu, Peoples R China

关键词： Pseudobagrus ussuriensis; Ulcerative syndrome; Pathogen; Transcriptome; Immune responses

期刊名称：AQUACULTURE （ 影响因子：3.224； 五年影响因子：3.591 ）

ISSN： 0044-8486

年卷期： 2021 年 537 卷

页码：

收录情况： SCI

摘要： Though ulcerative syndrome is the dominant disease in aquaculture of the Ussuri catfish (Pseudobagrus ussuriensis), available information for the disease in this fish is seriously limited. In the present study, the pathogen of ulcerative syndrome in P. ussuriensis was isolated and identified as Aeromonas veronii for the first time. To gain insight into the immune response against ulcerative syndrome, comparative transcriptome analyses of the liver, head kidney and spleen were performed between infected and healthy P. ussuriensis. A total of 73,772 unigenes with a mean length of 1172 bp were generated, and 44,996 (60.99%) were successfully annotated in public databases. Differentially expressed genes (DEGs) between infected and control groups were detected: 413 were identified in the liver (224 up-regulated and 189 down-regulated), 691 in the head kidney (225 up-regulated and 466 down-regulated), and 445 in the spleen (262 up-regulated and 183 down-regulated). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that DEGs were classified into 17 GO categories and 40 immune-related KEGG pathways in the liver, 36 GO categories and 45 immune-related pathways in the head kidney, and 39 categories and 38 immune-related pathways in the spleen. Through GO and KEGG annotation, 237 immune-related DEGs (115 up-regulated and 122 down-regulated) involving 212 annotated genes were identified. The DEGs reflected both innate and adaptive immunity responses against ulcerative syndrome. Twenty-six selected DEGs were successfully validated through real-time quantitative PCR. The results of this study provide basic information on immune responses of the liver, head kidney and spleen during ulcerative syndrome. This information will be useful for future genetic studies on the molecular mechanisms of the immune system and helpful for genetic improvement of P. ussuriensis against ulcerative syndrome.

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