Sphingopyxis microcysteis sp. nov., a novel bioactive exopolysaccharides-bearing Sphingomonadaceae isolated from the Microcystis phycosphere
文献类型： 外文期刊
作者： Zhang, Xiao-ling 1 ; Li, Gui-xian 1 ; Ge, Ya-ming 2 ; Iqbal, Nurhezreen Md 3 ; Yang, Xi 4 ; Cui, Zhen-dong 5 ; Yang, Q 1 ;
作者机构： 1.Zhejiang Ocean Univ, Dept Marine Chem, Zhoushan, Peoples R China
2.Zhejiang Ocean Univ, Natl Engn Res Ctr Marine Aquaculture, Zhoushan, Peoples R China
3.Natl Inst Biotechnol Malaysia, Malaysia Genome Inst, Kajang, Malaysia
4.Guangdong Acad Agr Sci, Inst Anim Sci, Guangzhou, Peoples R China
5.Yantai Univ, Sch Comp & Control Engn, Yantai, Peoples R China
6.Zhejiang Ocean Univ, ABI Grp, GPM Project, Zhoushan, Peoples R China
关键词： Sphingopyxis microcysteis sp; nov; Phycosphere microbiota; Bioflocculating exopolysaccharides; Microcystis aeruginosa; Toxic cyanobacteria; CyanoHAB
期刊名称：ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY （ 影响因子：2.674； 五年影响因子：2.09 ）
ISSN： 0003-6072
年卷期： 2021 年 114 卷 6 期
页码：
收录情况： SCI
摘要： During the study into the microbial biodiversity and bioactivity of the Microcystis phycosphere, a new yellow-pigmented, non-motile, rod-shaped bacterium containing polyhydroxybutyrate granules designated as strain Z10-6(T) was isolated from highly-toxic Microcystis aeruginosa Kutzing M.TN-2. The new isolate produces active bioflocculating exopolysaccharides. Phylogenetic analysis based on 16S rRNA gene sequences indicated strain Z10-6(T) belongs to the genus Sphingopyxis with highest similarity to Sphingopyxis solisilvae R366(T) (98.86%), and the similarity to other Sphingopyxis members was less than 98.65%. However, both low values obtained by phylogenomic calculation of average nucleotide identity (ANI, 85.5%) and digital DNA-DNA hybridization (dDDH, 29.8%) separated the new species from its closest relative. The main polar lipids were sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified glycolipid and one unidentified aminophospholipid. The predominant fatty acids were summed feature 8, C-17:1 omega 6c, summed feature 3, C-16:0, C-18:1 omega 7c 11-methyl and C-14:0 2-OH. The respiratory quinone was ubiqunone-10, with spermidine as the major polyamine. The genomic DNA G + C content was 64.8 mol%. Several biosynthesis pathways encoding for potential new bacterial bioactive metabolites were found in the genome of strain Z10-6(T). The polyphasic analyses clearly distinguished strain Z10-6(T) from its closest phylogenetic neighbors. Thus, it represents a novel species of the genus Sphingopyxis, for which the name Sphingopyxis microcysteis sp. nov. is proposed. The type strain is Z10-6(T) (= CCTCC AB2017276(T) = KCTC 62492(T)).
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