文献类型： 外文期刊
作者： Mori, Hirotada 1 ; Kataoka, Masakazu 2 ; Yang, Xi 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Sci, Innovat Lab Syst Microbiol & Synthet Biol, Guangzhou 510640, Peoples R China
2.Shinshu Univ, Fac Engn, Dept Environm Sci & Technol, Nagano 3908621, Japan
关键词： Escherichia coli K-12; mutation; homologous recombination; HR; site-specific recombination; genome modification; lambda Red; P1 transduction; recombineering
期刊名称：MICROORGANISMS （ 影响因子：4.926； 五年影响因子：5.143 ）
ISSN：
年卷期： 2022 年 10 卷 9 期
页码：
收录情况： SCI
摘要： Escherichia coli K-12 is one of the most well-studied species of bacteria. This species, however, is much more difficult to modify by homologous recombination (HR) than other model microorganisms. Research on HR in E. coli has led to a better understanding of the molecular mechanisms of HR, resulting in technical improvements and rapid progress in genome research, and allowing whole-genome mutagenesis and large-scale genome modifications. Developments using lambda Red (exo, bet, and gam) and CRISPR-Cas have made E. coli as amenable to genome modification as other model microorganisms, such as Saccharomyces cerevisiae and Bacillus subtilis. This review describes the history of recombination research in E. coli, as well as improvements in techniques for genome modification by HR. This review also describes the results of large-scale genome modification of E. coli using these technologies, including DNA synthesis and assembly. In addition, this article reviews recent advances in genome modification, considers future directions, and describes problems associated with the creation of cells by design.
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