文献类型： 外文期刊

作者： Tsukimi, Tomoya ¹ ; Obana, Nozomu ³ ; Shigemori, Suguru ³ ; Arakawa, Kazuharu ¹ ; Miyauchi, Eiji ⁵ ; Yang, Jiayue ¹ ; Song, Isaiah ¹ ; Ashino, Yujin ¹ ; Wakayama, Masataka ¹ ; Soga, Tomoyoshi ¹ ; Tomita, Masaru ¹ ; Ohno, Hiroshi ⁵ ; Mori, Hirotada ⁷ ; Fukuda, Shinji ¹ ;

作者机构： 1.Keio Univ, Inst Adv Biosci, Tsuruoka, Japan

2.Keio Univ, Grad Sch Media & Governance, Syst Biol Program, Fujisawa, Japan

3.Univ Tsukuba, Inst Med, Transborder Med Res Ctr, Tsukuba, Japan

4.Keio Univ, Fac Environm & Informat Studies, Fujisawa, Japan

5.RIKEN Ctr Integrat Med Sci, Yokohama, Japan

6.Gunma Univ, Inst Mol & Cellular Regulat, Maebashi, Japan

7.Nara Inst Sci & Technol, Grad Sch Biol Sci, Ikoma, Japan

8.Guangdong Acad Agr Sci, Inst Anim Sci, Guangzhou, Peoples R China

9.Kanagawa Inst Ind Sci & Technol, Gut Environm Design Grp, Kawasaki, Japan

10.Juntendo Univ, Lab Regenerat Microbiol, Grad Sch Med, Tokyo, Japan

11.Shinshu Univ, Inst Biomed Sci, Kami Ina, Japan

12.Ehime Univ, Integrated Med & Agr Sch Publ Hlth, Toon, Japan

关键词： gut microbiota; Escherichia coli; genetic mutation; intestinal colonization; intestinal nutrient

期刊名称：MSYSTEMS （ 影响因子：6.4； 五年影响因子：7.3 ）

ISSN： 2379-5077

年卷期： 2024 年 9 卷 2 期

页码：

收录情况： SCI

摘要： Mammalian gut microbes colonize the intestinal tract of their host and adapt to establish a microbial ecosystem. The host diet changes the nutrient profile of the intestine and has a high impact on microbiota composition. Genetic mutations in Escherichia coli, a prevalent species in the human gut, allow for adaptation to the mammalian intestine, as reported in previous studies. However, the extent of colonization fitness in the intestine elevated by genetic mutation and the effects of diet change on these mutations in E. coli are still poorly known. Here, we show that notable mutations in sugar metabolism-related genes (gatC, araC, and malI) were detected in the E. coli K-12 genome just 2 weeks after colonization in the germ-free mouse intestine. In addition to elevated fitness by deletion of gatC, as previously reported, deletion of araC and malI also elevated E. coli fitness in the murine intestine in a host diet-dependent manner. In vitro cultures of medium containing nutrients abundant in the intestine (e.g., galactose, N-acetylglucosamine, and asparagine) also showed increased E. coli fitness after deletion of the genes-of-interest associated with their metabolism. Furthermore, the host diet was found to influence the developmental trajectory of gene mutations in E. coli. Taken together, we suggest that genetic mutations in E. coli are selected in response to the intestinal environment, which facilitates efficient utilization of nutrients abundant in the intestine under laboratory conditions. Our study offers some insight into the possible adaptation mechanisms of gut microbes.